{"id":"W4387794530","doi":"10.1016/j.cbpa.2023.102403","title":"Strategies for quantifying the enzymatic activities of glycoside hydrolases within cells and in vivo","year":2023,"lang":"en","type":"review","venue":"Current Opinion in Chemical Biology","topic":"Glycosylation and Glycoproteins Research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":5,"is_retracted":false,"has_abstract":false,"ca_institutions":"Simon Fraser University","funders":"Canadian Glycomics Network; Canadian Institutes of Health Research; Natural Sciences and Engineering Research Council of Canada; Canada Research Chairs; Michael Smith Health Research BC; Michael J. Fox Foundation for Parkinson's Research","keywords":"Enzyme; Modalities; Computational biology; Biology; In vivo; Glycoside hydrolase; Biochemistry; Biotechnology","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004120607,0.0002450566,0.0006820632,0.0001675269,0.00002812199,0.00002176505,0.0002746423,0.0003531753,0.000005886882],"category_scores_gemma":[0.0002397272,0.00016991,0.0001537338,0.0002100822,0.0002611444,0.000004736749,0.000206228,0.0002997517,0.00000180489],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002278739,"about_ca_system_score_gemma":0.0002384619,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001563688,"about_ca_topic_score_gemma":0.00002483456,"domain_scores_codex":[0.9984035,0.0002017024,0.0006228681,0.0004134812,0.00008077925,0.000277592],"domain_scores_gemma":[0.9990155,0.0004311629,0.0002402459,0.0002444006,0.00002986452,0.00003880674],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"design_other","study_design_gemma":"not_applicable","study_design_scores_codex":[0.0005336496,0.0005231674,0.0002195744,0.1444883,0.000323871,0.000001418931,0.0004728319,0.00006094449,0.2937908,0.005751953,0.003182502,0.550651],"study_design_scores_gemma":[0.0007472533,0.0002013925,0.00000185518,0.007477442,0.00003340897,0.000009373333,0.0003205421,0.0002373228,0.053815,0.001065505,0.9356537,0.0004372034],"study_design_candidate":"not_applicable","study_design_consensus":null,"genre_codex":"review","genre_gemma":"review","genre_scores_codex":[0.009044853,0.9887632,0.00008857366,0.00002399196,0.0006639836,0.001148436,0.0002363497,0.000008680085,0.00002200756],"genre_scores_gemma":[0.007613174,0.9912558,0.00007405533,0.000003516287,0.00017156,0.000495611,0.0003444229,0.00002654646,0.0000152812],"genre_candidate":"review","genre_consensus":"review","teacher_disagreement_score":0.9324712,"threshold_uncertainty_score":0.6928728,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.1881068556599983,"score_gpt":0.4471799052199702,"score_spread":0.2590730495599719,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}