{"id":"W4387968640","doi":"10.3791/65382","title":"<em>Agrobacterium tumefaciens</em>-Mediated Genetic Engineering of Green Microalgae, <em>Chlorella vulgaris</em>","year":2023,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"Algal biology and biofuel production","field":"Energy","cited_by":3,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Waterloo","funders":"","keywords":"Chlorella vulgaris; Agrobacterium tumefaciens; Biology; Transformation (genetics); Expression vector; Agrobacterium; Genetically modified crops; Genome; Gene; Expression cassette; Reporter gene; Transgene; Genetics; Botany; Vector (molecular biology); Gene expression; Algae","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.001474383,0.001061002,0.001688344,0.001152571,0.0003394635,0.0001686475,0.001121308,0.001123832,0.0008343791],"category_scores_gemma":[0.0004906563,0.0009526649,0.0007633101,0.001508326,0.0002439397,0.0005418024,0.000463945,0.001014688,0.0006043449],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004178331,"about_ca_system_score_gemma":0.0002245087,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00009760966,"about_ca_topic_score_gemma":0.00006452545,"domain_scores_codex":[0.9928867,0.000513415,0.002666827,0.001028196,0.001411121,0.001493774],"domain_scores_gemma":[0.9957539,0.0002127421,0.001736018,0.0008761508,0.0008022758,0.0006189481],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.001226414,0.0005963914,0.0009043009,0.0001660365,0.001023233,0.0002501177,0.003906247,0.0006432684,0.983939,0.0001360555,0.004855112,0.002353805],"study_design_scores_gemma":[0.008929432,0.00229886,0.03282521,0.0005463429,0.0005164476,0.0007169869,0.001223319,0.002728812,0.8801377,0.0002853226,0.06777333,0.002018211],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9895588,0.004766993,0.0002726369,0.0001754736,0.004040122,0.00051182,0.0000768218,0.0002838529,0.0003134344],"genre_scores_gemma":[0.9901684,0.001697522,0.00279006,0.0002913417,0.001761658,0.00005025342,0.0003092756,0.0002370844,0.00269442],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1038013,"threshold_uncertainty_score":0.9992924,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01823276912341418,"score_gpt":0.2979055691951664,"score_spread":0.2796728000717522,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}