{"id":"W4387968640","doi":"10.3791/65382","title":"&lt;em&gt;Agrobacterium tumefaciens&lt;/em&gt;-Mediated Genetic Engineering of Green Microalgae, &lt;em&gt;Chlorella vulgaris&lt;/em&gt;","year":2023,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"Algal biology and biofuel production","field":"Energy","cited_by":3,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Waterloo","funders":"","keywords":"Chlorella vulgaris; Agrobacterium tumefaciens; Biology; Transformation (genetics); Expression vector; Agrobacterium; Genetically modified crops; Genome; Gene; Expression cassette; Reporter gene; Transgene; Genetics; Botany; Vector (molecular biology); Gene expression; Algae","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003771161,0.0007852898,0.0004280693,0.000574186,0.0003280052,0.0003864998,0.0005523663,0.0003643467,0.002361149],"category_scores_gemma":[0.0002295179,0.0003175675,0.0003579891,0.000676682,0.0002966108,0.000271188,0.0004037593,0.0007512763,0.003815119],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004266737,"about_ca_system_score_gemma":0.0004443013,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001381721,"about_ca_topic_score_gemma":0.002722325,"domain_scores_codex":[0.9997286,0.00004177229,0.00002897453,0.00006098071,0.0001075643,0.00003202627],"domain_scores_gemma":[0.9998908,0.00001466856,0.00003494315,0.00002319415,0.00001723271,0.00001913755],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003549588,0.00001674414,0.00005955171,0.00007367083,0.000004425708,0.00005779458,0.00001070505,0.00004507657,0.9949971,0.0001901644,0.0004675879,0.004041783],"study_design_scores_gemma":[0.00001801759,0.00007781717,0.002077488,0.00002108703,0.00002294373,0.0004334821,0.00002710056,0.0007079843,0.9592091,0.0001333737,0.0372557,0.00001577798],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.539256,0.007877517,0.377348,0.001730752,0.0007973985,0.001743299,0.02296807,0.01126429,0.03701453],"genre_scores_gemma":[0.5799515,0.007185888,0.2768483,0.0006258919,0.0000679638,0.001183878,0.0533752,0.001978876,0.07878261],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002361149,"threshold_uncertainty_score":0.007898808,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01823276912341418,"score_gpt":0.2979055691951664,"score_spread":0.2796728000717522,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}