{"id":"W4388214118","doi":"10.26508/lsa.202302045","title":"CRISPR/Cas9 bioluminescence-based assay for monitoring CFTR trafficking to the plasma membrane","year":2023,"lang":"en","type":"article","venue":"Life Science Alliance","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":1,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University; McGill University Health Centre","funders":"Institut de recherche, Centre universitaire de santé McGill; University of North Carolina at Chapel Hill; Canadian Institutes of Health Research; McGill University Health Centre; Ministère du Développement Économique, de l’Innovation et de l’Exportation; Cystic Fibrosis Foundation Therapeutics","keywords":"CRISPR; Cas9; Genome editing; Luciferase; Molecular biology; Bioluminescence; Biology; HEK 293 cells; Transfection; Chemistry; Cell biology; Gene; Genetics; Biochemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0008478307,0.0001468256,0.0001080097,0.00006265671,0.0003344741,0.00009749707,0.0006908874,0.00005801611,0.000002536144],"category_scores_gemma":[0.0007270504,0.0001196192,0.00007035307,0.0008976366,0.0001344753,0.000008260656,0.00009767107,0.00006661623,0.00003681847],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001617904,"about_ca_system_score_gemma":0.0001629412,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000005657365,"about_ca_topic_score_gemma":0.00001269103,"domain_scores_codex":[0.9985012,0.00001484744,0.0001697874,0.0005000049,0.0002802321,0.0005338666],"domain_scores_gemma":[0.9991919,0.00005472808,0.00003849557,0.0004280656,0.0001105257,0.0001762539],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002096913,0.000009955572,0.001213772,0.00002516426,0.000006059118,0.00000107074,0.00009547174,0.06829613,0.9236676,0.00001899972,0.001689167,0.004955715],"study_design_scores_gemma":[0.0002280839,0.00009470872,0.004277273,0.00004259168,0.000006226785,0.000001518036,0.0001850577,0.02613625,0.9257445,0.000002742394,0.04307177,0.0002092946],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9426808,0.0002940904,0.0521119,0.002664872,0.001521028,0.0005086135,0.00001846915,0.00008622537,0.0001139906],"genre_scores_gemma":[0.9939306,0.00005922069,0.00448639,0.0003130628,0.0006466785,0.0001185323,0.000008616751,0.00001997488,0.0004169195],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.05124979,"threshold_uncertainty_score":0.4877929,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02506300335222427,"score_gpt":0.3343769760716804,"score_spread":0.3093139727194562,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}