{"id":"W4388304697","doi":"10.3390/pathogens12111314","title":"Optimization of a Protocol for Launching Grapevine Infection with the Biologically Active cDNA Clones of a Virus","year":2023,"lang":"en","type":"article","venue":"Pathogens","topic":"Plant Virus Research Studies","field":"Agricultural and Biological Sciences","cited_by":8,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Guelph","funders":"","keywords":"Infectivity; Biology; Complementary DNA; Virus; Virology; cDNA library; RNA extraction; RNA; Agroinfiltration; Nicotiana benthamiana; Gene; Genetics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001853207,0.0012313,0.001343693,0.001068708,0.000832779,0.0009491036,0.0008576502,0.001019215,0.002377005],"category_scores_gemma":[0.001108505,0.00092394,0.001326788,0.0006437669,0.0007600359,0.0006375572,0.0008632836,0.003181245,0.002070601],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006219465,"about_ca_system_score_gemma":0.0009751195,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001295641,"about_ca_topic_score_gemma":0.002846738,"domain_scores_codex":[0.9979433,0.0004429548,0.0003676348,0.0005727329,0.0004467351,0.0002266957],"domain_scores_gemma":[0.9990566,0.0003008187,0.0001399106,0.0002309378,0.000167698,0.0001039541],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004399973,0.00007762788,0.0001561305,0.000154493,0.00001161832,0.00007652138,0.0000858165,0.00012729,0.9968609,0.0001627345,0.0001961231,0.002046887],"study_design_scores_gemma":[0.00006357394,0.001003073,0.004287523,0.0000548608,0.000119293,0.0005894398,0.00009411744,0.001973204,0.970041,0.0002329551,0.02145505,0.00008579885],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.5784118,0.004767939,0.3738968,0.0007429986,0.001510305,0.01878731,0.007181356,0.003656762,0.01104462],"genre_scores_gemma":[0.3723268,0.003553101,0.5716336,0.0006392148,0.0001740759,0.01319369,0.02125962,0.001533775,0.01568617],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.002377005,"threshold_uncertainty_score":0.009800851,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.06674995374974872,"score_gpt":0.3117486931732824,"score_spread":0.2449987394235337,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}