{"id":"W4388558302","doi":"10.1139/bcb-2023-0231","title":"Optimized total RNA isolation from bovine sperm with enhanced sperm head lysis","year":2023,"lang":"en","type":"article","venue":"Biochemistry and Cell Biology","topic":"Molecular Biology Techniques and Applications","field":"Biochemistry, Genetics and Molecular Biology","cited_by":3,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Lethbridge; University of Calgary","funders":"Natural Sciences and Engineering Research Council of Canada; Government of Alberta","keywords":"Sperm; TCEP; Lysis; RNA; Trizol; RNA extraction; Semen; Andrology; Protamine; Lysis buffer; Biology; Human fertilization; Intracytoplasmic sperm injection; Chemistry; Molecular biology; Cell biology; Biochemistry; In vitro fertilisation; Anatomy; Genetics; Embryo; Gene; Medicine","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":true,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001872162,0.001164322,0.0009886605,0.001167113,0.0006672395,0.0008385917,0.000868729,0.0008104136,0.002209511],"category_scores_gemma":[0.001351287,0.000528849,0.0007274775,0.0009574289,0.0006237312,0.0004148392,0.0006526231,0.001391426,0.0025972],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004099329,"about_ca_system_score_gemma":0.001035289,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0009539338,"about_ca_topic_score_gemma":0.003960988,"domain_scores_codex":[0.9976935,0.0003806259,0.0003268796,0.0006503237,0.0007230106,0.0002257463],"domain_scores_gemma":[0.9992854,0.0001950955,0.0001315486,0.00009640746,0.000252778,0.00003886494],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002425136,0.00001510199,0.00006227507,0.0001427133,0.000005458938,0.00003886345,0.00002499313,0.00009525284,0.9971015,0.00007072719,0.0001248657,0.002293951],"study_design_scores_gemma":[0.0000271945,0.0004385092,0.00296445,0.00005970664,0.00007453706,0.0003867675,0.00003988262,0.001828523,0.9704053,0.0001580296,0.0235692,0.00004787672],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.2713584,0.01362912,0.6802505,0.0006496627,0.001208098,0.004876021,0.01392502,0.004231072,0.00987224],"genre_scores_gemma":[0.2128087,0.007494099,0.7253867,0.0006396284,0.0003200768,0.003993195,0.0332218,0.001269989,0.01486588],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.002209511,"threshold_uncertainty_score":0.009901106,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.00593134346773768,"score_gpt":0.2416037340128713,"score_spread":0.2356723905451336,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}