{"id":"W4388672331","doi":"10.3390/biomedicines11113050","title":"Transient Expression in HEK-293 Cells in Suspension Culture as a Rapid and Powerful Tool: SARS-CoV-2 N and Chimeric SARS-CoV-2N-CD154 Proteins as a Case Study","year":2023,"lang":"en","type":"article","venue":"Biomedicines","topic":"SARS-CoV-2 and COVID-19 Research","field":"Medicine","cited_by":1,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University","funders":"McGill University","keywords":"HEK 293 cells; Fusion protein; Transfection; Molecular biology; Antigen; clone (Java method); Biology; Cell culture; Chemistry; Recombinant DNA; Biochemistry; Immunology; Gene; Genetics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.001038169,0.0005177464,0.0009092216,0.001429432,0.0001366014,0.00006437543,0.0001778567,0.0002939664,0.000008511849],"category_scores_gemma":[0.0003060572,0.0003732475,0.00008920122,0.002441647,0.0003589855,0.0001997107,0.0002071896,0.0005782057,0.00008669488],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000115897,"about_ca_system_score_gemma":0.0002536611,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.004531884,"about_ca_topic_score_gemma":0.001069149,"domain_scores_codex":[0.9962339,0.000272159,0.0007349679,0.001048692,0.0009199489,0.0007902686],"domain_scores_gemma":[0.998915,0.0001664204,0.0001182189,0.0005702842,0.0001349669,0.00009511209],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.001116444,0.0009048044,0.01043312,0.0003736623,0.00004209025,0.02183857,0.0141537,7.042846e-8,0.9435523,0.000002119447,0.002846842,0.004736354],"study_design_scores_gemma":[0.01405199,0.00507653,0.0110258,0.001316398,0.00009880341,0.003087922,0.01755746,0.0002869825,0.9079041,0.00007421375,0.03896297,0.0005567631],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9922456,0.001994106,0.000007227932,0.001150428,0.0002407641,0.003804172,0.00001214742,0.0001527657,0.0003927818],"genre_scores_gemma":[0.9927047,0.0002020012,0.00008866221,0.006439383,0.0001982337,0.0001994185,0.00001425871,0.00006909107,0.00008420372],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.03611612,"threshold_uncertainty_score":0.999872,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0471937170619088,"score_gpt":0.3646586220737454,"score_spread":0.3174649050118366,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}