{"id":"W4388680084","doi":"10.1101/2023.11.11.566649","title":"Proteome-wide base editor screens to assess phosphorylation site functionality in high-throughput","year":2023,"lang":"en","type":"preprint","venue":"bioRxiv (Cold Spring Harbor Laboratory)","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":1,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"","keywords":"Phosphoproteomics; NFAT; Phosphorylation; Biology; Computational biology; Proteome; Transcription factor; Phenotype; Signal transduction; Function (biology); Gene; Cell biology; Protein phosphorylation; Genetics; Protein kinase A","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0009806663,0.000836541,0.0006406435,0.0005883488,0.0004596977,0.0008522666,0.000648686,0.0006290771,0.003100842],"category_scores_gemma":[0.001004434,0.0003158766,0.0004685031,0.0005540021,0.0004461629,0.0003812608,0.0007061552,0.00117012,0.001082438],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003790688,"about_ca_system_score_gemma":0.0003137074,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0003463692,"about_ca_topic_score_gemma":0.001175261,"domain_scores_codex":[0.9994189,0.00009102743,0.0000654845,0.0001242059,0.0002373543,0.0000630305],"domain_scores_gemma":[0.9990891,0.0003740937,0.0002303573,0.0001503538,0.00006415676,0.00009185308],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001205552,0.0000414208,0.000345578,0.00005938446,0.00002182419,0.0001496967,0.0000175038,0.0003973553,0.995109,0.0002593077,0.0002267551,0.003251636],"study_design_scores_gemma":[0.0000184528,0.00009347292,0.001444255,0.000004098212,0.00002563362,0.0002813157,0.00001451476,0.00176455,0.9933683,0.0001928966,0.002780536,0.00001198716],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8389204,0.001317897,0.1378856,0.0005237419,0.000248173,0.0004585999,0.007373794,0.005591868,0.007679937],"genre_scores_gemma":[0.9110767,0.001108558,0.07375071,0.000372232,0.00004210094,0.0003470093,0.005396058,0.00165349,0.006253216],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.003100842,"threshold_uncertainty_score":0.01037335,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01827859643517177,"score_gpt":0.2648798298902775,"score_spread":0.2466012334551057,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}