{"id":"W4388728648","doi":"10.1038/s41589-023-01483-3","title":"Mechanistic plasticity in ApmA enables aminoglycoside promiscuity for resistance","year":2023,"lang":"en","type":"article","venue":"Nature Chemical Biology","topic":"Antibiotic Resistance in Bacteria","field":"Biochemistry, Genetics and Molecular Biology","cited_by":10,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Calgary; University of Toronto; McMaster University","funders":"National Institute of Allergy and Infectious Diseases; Canadian Institutes of Health Research; Canada Research Chairs; U.S. Department of Health and Human Services; Government of Canada; Ontario Research Foundation","keywords":"Active site; Enzyme; Biology; Histidine; Biochemistry; Aminoglycoside; Chemistry; Genetics; Computational biology; Antibiotics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001878141,0.0002099404,0.0002814162,0.00006338856,0.00004870936,0.00001327317,0.0003443103,0.0009841992,0.00001166341],"category_scores_gemma":[0.001689865,0.0001873918,0.00009405727,0.0002194087,0.0001663114,0.000002797786,0.0001452002,0.0003057044,0.0000143397],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0000428114,"about_ca_system_score_gemma":0.00006826403,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000003100883,"about_ca_topic_score_gemma":0.0000862907,"domain_scores_codex":[0.9984408,0.00004814516,0.0002841505,0.0006429158,0.00007402487,0.000509935],"domain_scores_gemma":[0.9993222,0.0001630274,0.00008828955,0.0002700697,0.00008244858,0.000073978],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0004432655,0.00003690713,0.002937633,0.00008315587,0.00002138415,0.000005254533,0.000006266986,0.00000101235,0.9884018,0.003463593,0.004417913,0.000181826],"study_design_scores_gemma":[0.0007885487,0.00009066777,0.003170425,0.00003707387,0.0000125052,0.000004173432,0.00001642147,0.00004658638,0.9477481,0.008789093,0.03901158,0.0002848652],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9968593,0.000292178,0.001091975,0.0002602586,0.0002825304,0.000416913,0.0001756027,0.00005237645,0.0005688792],"genre_scores_gemma":[0.995535,0.00008093896,0.002235165,0.0002253624,0.0003077718,0.00006631566,0.0007050525,0.00002725833,0.000817143],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.04065373,"threshold_uncertainty_score":0.7641616,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.00966087250680871,"score_gpt":0.2800372975105862,"score_spread":0.2703764250037775,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}