{"id":"W4388861783","doi":"10.1007/978-1-0716-3485-1_10","title":"Monitoring the Intracellular Trafficking of Virus-Induced Structures and Intercellular Spread of Viral Infection in Plants Using Endomembrane Trafficking Pathway-Specific Chemical Inhibitor and Organelle-Selective Fluorescence Dye","year":2023,"lang":"en","type":"article","venue":"Methods in molecular biology","topic":"Plant Virus Research Studies","field":"Agricultural and Biological Sciences","cited_by":0,"is_retracted":false,"has_abstract":false,"ca_institutions":"Agriculture and Agri-Food Canada","funders":"","keywords":"Endomembrane system; Cell biology; Biology; Endosome; Golgi apparatus; Endoplasmic reticulum; Plasmodesma; Viral replication; Endocytic cycle; Endocytosis; Intracellular; Virology; Biochemistry; Virus; Cell; Cytoplasm","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001494135,0.0002699626,0.000232155,0.0001544118,0.0002446178,0.0002646942,0.0003391213,0.000418003,0.0005400983],"category_scores_gemma":[0.0001256811,0.0001936752,0.000218659,0.0001867633,0.000227979,0.0004056293,0.000191604,0.001166412,0.0001767879],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004802164,"about_ca_system_score_gemma":0.00024731,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001182197,"about_ca_topic_score_gemma":0.001099834,"domain_scores_codex":[0.9999137,0.0000116839,0.000003359148,0.00003166929,0.00001715569,0.00002249688],"domain_scores_gemma":[0.9998614,0.00005230388,0.00002936104,0.00001348733,0.00002025658,0.00002319825],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002285025,0.000005976742,0.00005306786,0.00001082211,0.00000116257,0.000007574948,0.00001022555,0.00002935021,0.9995459,0.0000701362,0.0000066757,0.0002361773],"study_design_scores_gemma":[0.000007603858,0.00005498763,0.001693451,0.000002542741,0.000005989993,0.0000464466,0.00001834893,0.001783427,0.9960234,0.00003807971,0.0003219304,0.000003849789],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.9797505,0.0009835577,0.01741672,0.00007404167,0.00001515589,0.00003093022,0.0001741471,0.00009556763,0.001459408],"genre_scores_gemma":[0.9801379,0.001042091,0.01554,0.00006767147,0.00000913698,0.0001290451,0.0004224738,0.00004927948,0.002602514],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.001182197,"threshold_uncertainty_score":0.003484249,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.05381205080688258,"score_gpt":0.3378246694546648,"score_spread":0.2840126186477822,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}