{"id":"W4389136684","doi":"10.1128/spectrum.03692-23","title":"CRISPR-Cas9-mediated IncF plasmid curing in extraintestinal pathogenic <i>Escherichia coli</i>","year":2023,"lang":"en","type":"article","venue":"Microbiology Spectrum","topic":"Antibiotic Resistance in Bacteria","field":"Biochemistry, Genetics and Molecular Biology","cited_by":4,"is_retracted":false,"has_abstract":true,"ca_institutions":"Calgary Laboratory Services; University of Calgary","funders":"NHLBI Division of Intramural Research; National Institute on Alcohol Abuse and Alcoholism; Joint Programming Initiative on Antimicrobial Resistance; Canadian Institutes of Health Research; National Institutes of Health; Government of Canada; National Institute of Allergy and Infectious Diseases; Division of Intramural Research, National Institute of Allergy and Infectious Diseases","keywords":"Plasmid; Biology; CRISPR; Virulence; Microbiology; Escherichia coli; Antibiotic resistance; Genetics; Gene; Antibiotics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002283065,0.0003389912,0.0003428219,0.000177314,0.0001417379,0.0003077824,0.0003277849,0.0003566679,0.001171838],"category_scores_gemma":[0.0002810128,0.0001455981,0.0003190876,0.0001146568,0.0002256316,0.0002007561,0.0003595801,0.0006297808,0.0004129766],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002117509,"about_ca_system_score_gemma":0.0002904096,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0009901758,"about_ca_topic_score_gemma":0.00126735,"domain_scores_codex":[0.9996898,0.00004766717,0.00004171187,0.00007274922,0.00008747634,0.00006058453],"domain_scores_gemma":[0.9997616,0.00005052378,0.0000795172,0.00004656857,0.00002880736,0.00003295304],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004228241,0.00003076207,0.0003110385,0.00005458361,0.00000538117,0.00006229822,0.00001671437,0.000134727,0.9967295,0.0001107921,0.0001015466,0.002400375],"study_design_scores_gemma":[0.000007183213,0.0001692908,0.003271753,0.000007668705,0.00001221814,0.000308851,0.00002469608,0.002101826,0.9908502,0.00003661368,0.003199571,0.00001009549],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9699454,0.0006722329,0.02479331,0.0001328497,0.00007446246,0.0001208662,0.0008349813,0.0005937755,0.002832111],"genre_scores_gemma":[0.977698,0.0003438934,0.01646932,0.0001066305,0.000007936647,0.0000757989,0.001181441,0.000113516,0.004003378],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001171838,"threshold_uncertainty_score":0.003920138,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008744830649809712,"score_gpt":0.2331252848683754,"score_spread":0.2243804542185657,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}