{"id":"W4389136684","doi":"10.1128/spectrum.03692-23","title":"CRISPR-Cas9-mediated IncF plasmid curing in extraintestinal pathogenic <i>Escherichia coli</i>","year":2023,"lang":"en","type":"article","venue":"Microbiology Spectrum","topic":"Antibiotic Resistance in Bacteria","field":"Biochemistry, Genetics and Molecular Biology","cited_by":4,"is_retracted":false,"has_abstract":true,"ca_institutions":"Calgary Laboratory Services; University of Calgary","funders":"NHLBI Division of Intramural Research; National Institute on Alcohol Abuse and Alcoholism; Joint Programming Initiative on Antimicrobial Resistance; Canadian Institutes of Health Research; National Institutes of Health; Government of Canada; National Institute of Allergy and Infectious Diseases; Division of Intramural Research, National Institute of Allergy and Infectious Diseases","keywords":"Plasmid; Biology; CRISPR; Virulence; Microbiology; Escherichia coli; Antibiotic resistance; Genetics; Gene; Antibiotics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.0003243686,0.0002876893,0.0002896853,0.0001445162,0.00008420058,0.00002257667,0.000349021,0.0003399698,0.0001040462],"category_scores_gemma":[0.0001760298,0.0002954636,0.0001060612,0.0003979316,0.0002586377,0.000005201523,0.0002412966,0.0002706136,0.0004129529],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00005055758,"about_ca_system_score_gemma":0.0001228911,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00002975034,"about_ca_topic_score_gemma":0.0003346006,"domain_scores_codex":[0.9979745,0.0001421373,0.0004061231,0.0006619957,0.00004805773,0.0007671818],"domain_scores_gemma":[0.9993559,0.00005145363,0.0001197748,0.0003615786,0.00002793729,0.00008337399],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001281982,0.00004655172,0.06628642,0.00001882716,0.00003023123,0.00009599935,0.00003253713,0.00001736883,0.9292189,0.00003941275,0.004040788,0.00004474456],"study_design_scores_gemma":[0.001915456,0.0005017883,0.1955276,0.00008943014,0.00002548727,0.0002839461,0.000222342,0.00016695,0.7370803,0.0001805275,0.06308548,0.0009207124],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9974393,0.000291694,0.0001924575,0.0005640731,0.0004352485,0.0002450481,0.0001250143,0.000090064,0.000617079],"genre_scores_gemma":[0.9971038,0.0002262243,0.0004643035,0.0004462597,0.0002061689,0.00001610058,0.0008282603,0.00005046748,0.0006584821],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1921386,"threshold_uncertainty_score":0.9999498,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008744830649809712,"score_gpt":0.2331252848683754,"score_spread":0.2243804542185657,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}