{"id":"W4389192597","doi":"10.22215/etd/2023-15700","title":"Targeted deletion of repeat sequences in the 3’UTR of MDM2 using CRISPR/Cas9","year":2023,"lang":"en","type":"dissertation","venue":"","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"Carleton University","funders":"","keywords":"CRISPR; Biology; Mdm2; Ubiquitin ligase; Untranslated region; Gene; Genetics; Cas9; Five prime untranslated region; Messenger RNA; Molecular biology; Computational biology; Ubiquitin","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001007917,0.0001818214,0.0001364558,0.0001312246,0.000168716,0.0002078458,0.000155251,0.0002183145,0.001977067],"category_scores_gemma":[0.00007770909,0.0001085935,0.0002036032,0.00009770655,0.0001162639,0.0001019915,0.0002141575,0.0004565391,0.0009709691],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002382255,"about_ca_system_score_gemma":0.0002187254,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005623504,"about_ca_topic_score_gemma":0.001069274,"domain_scores_codex":[0.9999274,0.000005181559,0.000005301452,0.00002227315,0.00002529529,0.00001463034],"domain_scores_gemma":[0.9999585,0.000006986798,0.00001020914,0.000005315265,0.000006148077,0.0000127723],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002228969,0.000008498622,0.00008792736,0.00001974442,0.000001638067,0.00005285726,0.00001190258,0.00005774353,0.9972547,0.0001651694,0.00009967068,0.002217943],"study_design_scores_gemma":[0.000005201043,0.00007249392,0.001865887,0.000008306601,0.000006711203,0.0002121131,0.00001922538,0.0004723439,0.9875181,0.00006405771,0.009751869,0.000003716022],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9240826,0.002096517,0.04789509,0.0004092637,0.0001905602,0.000187608,0.001829158,0.000695192,0.02261407],"genre_scores_gemma":[0.9414392,0.001326172,0.02299365,0.0001529881,0.00001963577,0.0001092555,0.001951632,0.0001104087,0.03189711],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001977067,"threshold_uncertainty_score":0.00661391,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01595354355974039,"score_gpt":0.3475542535888738,"score_spread":0.3316007100291334,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}