{"id":"W4389230336","doi":"10.1182/blood-2023-185068","title":"Lipid Nanoparticle-Mediated Gene Editing of Human Primary T Cells and Off-Target Analysis of the CRISPR-Cas9 Indels","year":2023,"lang":"en","type":"article","venue":"Blood","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":6,"is_retracted":false,"has_abstract":true,"ca_institutions":"Precision Nanosystems (Canada)","funders":"","keywords":"CRISPR; Cas9; Genome editing; Electroporation; Biology; Computational biology; Molecular biology; Gene; Genetics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002923927,0.0003150573,0.0002354218,0.0001600207,0.0001613108,0.0002775487,0.0001924885,0.0003895784,0.0009838734],"category_scores_gemma":[0.0001547544,0.0001270333,0.0003030104,0.0001195576,0.000198445,0.0001163324,0.0001667704,0.0004945504,0.0004427909],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003466562,"about_ca_system_score_gemma":0.0001760025,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005824941,"about_ca_topic_score_gemma":0.0008104564,"domain_scores_codex":[0.9997569,0.00003432383,0.00002397293,0.00007561342,0.00007298392,0.00003631315],"domain_scores_gemma":[0.9998994,0.00002497872,0.00002008522,0.00001430168,0.00002582478,0.0000153341],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002882053,0.00001185907,0.00005416228,0.00001809868,0.000002418546,0.00002934503,0.00001089678,0.00009895261,0.9991884,0.00002183157,0.00002491005,0.0005104123],"study_design_scores_gemma":[0.000003175325,0.00006234473,0.0002698897,0.00000116614,0.000004290823,0.00006414734,0.000003637433,0.0005829,0.9986416,0.000008631536,0.0003566888,0.000001570782],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9557587,0.0007434321,0.03939436,0.0001270586,0.00005036542,0.0001599223,0.001009563,0.0003619321,0.002394664],"genre_scores_gemma":[0.9712964,0.000513475,0.0236454,0.00008077833,0.000009778258,0.0001414186,0.0009877889,0.00007574742,0.003249174],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0009838734,"threshold_uncertainty_score":0.003291368,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006419948274681695,"score_gpt":0.2510793971740688,"score_spread":0.2446594488993871,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}