{"id":"W4389323386","doi":"10.1021/acssynbio.3c00319","title":"<i>De Novo</i> Synthesis of a Conjugative System from Human Gut Metagenomic Data for Targeted Delivery of Cas9 Antimicrobials","year":2023,"lang":"en","type":"article","venue":"ACS Synthetic Biology","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":6,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université de Sherbrooke; Western University","funders":"Natural Sciences and Engineering Research Council of Canada; Canadian Institutes of Health Research","keywords":"Metagenomics; Computational biology; CRISPR; Biology; Contig; Plasmid; Cas9; Genetics; Escherichia coli; Citrobacter rodentium; Gene; Genome","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0008616265,0.000646678,0.0006768655,0.001024475,0.000437783,0.001080223,0.0003772283,0.0004615854,0.004507567],"category_scores_gemma":[0.00158749,0.000399141,0.0008582237,0.0009713346,0.0002649515,0.0003880561,0.0006774347,0.0009455884,0.003164945],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004155669,"about_ca_system_score_gemma":0.0006502396,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0006749076,"about_ca_topic_score_gemma":0.001452472,"domain_scores_codex":[0.9996231,0.00004860519,0.00004938582,0.0001250619,0.0001064519,0.00004745662],"domain_scores_gemma":[0.99926,0.0002164306,0.0001724779,0.0001433054,0.0001449065,0.00006288756],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0003207739,0.00003415889,0.003192384,0.0003891472,0.00005774627,0.0001989086,0.0001059611,0.0007539004,0.9755533,0.0004843244,0.001626752,0.01728267],"study_design_scores_gemma":[0.00002617031,0.000360027,0.01213692,0.00005063309,0.000104703,0.0006789451,0.000136265,0.007744466,0.9476774,0.0005714257,0.03046378,0.00004925612],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.5617141,0.002529647,0.3438476,0.001048834,0.0002592476,0.000775872,0.07654756,0.007363108,0.005913995],"genre_scores_gemma":[0.4236763,0.001919692,0.4602416,0.0004670484,0.00009051486,0.0006760927,0.1067313,0.001800829,0.004396637],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.004507567,"threshold_uncertainty_score":0.01507932,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0244908916941262,"score_gpt":0.3258282583754168,"score_spread":0.3013373666812906,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}