{"id":"W4389323386","doi":"10.1021/acssynbio.3c00319","title":"<i>De Novo</i> Synthesis of a Conjugative System from Human Gut Metagenomic Data for Targeted Delivery of Cas9 Antimicrobials","year":2023,"lang":"en","type":"article","venue":"ACS Synthetic Biology","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":6,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université de Sherbrooke; Western University","funders":"Natural Sciences and Engineering Research Council of Canada; Canadian Institutes of Health Research","keywords":"Metagenomics; Computational biology; CRISPR; Biology; Contig; Plasmid; Cas9; Genetics; Escherichia coli; Citrobacter rodentium; Gene; Genome","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004437255,0.0001753366,0.0004542766,0.00008247537,0.00004830986,0.000005313984,0.0005447847,0.0002293872,0.0000125058],"category_scores_gemma":[0.0002700999,0.0001689106,0.0001060161,0.00009747774,0.0001738072,0.000002558289,0.0003096519,0.0000484321,0.00000545039],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001275289,"about_ca_system_score_gemma":0.00006587421,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001989903,"about_ca_topic_score_gemma":0.0000289299,"domain_scores_codex":[0.9986655,0.0001102786,0.0004377715,0.0004522442,0.00004366648,0.000290502],"domain_scores_gemma":[0.9985995,0.0002515512,0.0001909651,0.0008213973,0.0000910603,0.00004556443],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005433107,0.0000253227,0.0002058908,0.0001247626,0.0003132437,0.000001550186,0.00005662346,0.0001458305,0.9975668,0.0001485215,0.0004767277,0.0008804459],"study_design_scores_gemma":[0.0003264288,0.0001325145,0.000363171,0.00005958627,0.0001705869,0.000009585119,0.0002208007,0.0003194925,0.9952882,0.00005143946,0.00288799,0.0001701877],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9874566,0.0008692989,0.00654609,0.0000454333,0.0001618108,0.0003022164,0.004503315,0.00002689002,0.00008830563],"genre_scores_gemma":[0.9962084,0.0001607002,0.002272816,0.00002456372,0.00008965812,0.00003960593,0.001136457,0.00003257521,0.00003520828],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.008751775,"threshold_uncertainty_score":0.6887975,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0244908916941262,"score_gpt":0.3258282583754168,"score_spread":0.3013373666812906,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}