{"id":"W4389799374","doi":"10.1101/pdb.prot108283","title":"Membrane Protein Fractionation and Analysis through Western Blot in<i>Aedes aegypti</i>Malpighian Tubules","year":2023,"lang":"en","type":"article","venue":"Cold Spring Harbor Protocols","topic":"Insect Resistance and Genetics","field":"Biochemistry, Genetics and Molecular Biology","cited_by":3,"is_retracted":false,"has_abstract":true,"ca_institutions":"York University","funders":"","keywords":"Western blot; Fractionation; Membrane protein; Cell fractionation; Blot; Biology; Cytosol; Biochemistry; Protein purification; Bacterial outer membrane; Chemistry; Membrane; Chromatography","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001007234,0.001028116,0.0007144868,0.001199895,0.001041142,0.0009639023,0.001018667,0.0007085598,0.003762549],"category_scores_gemma":[0.000369125,0.000616645,0.0008597485,0.001202004,0.0005783929,0.001071525,0.0005899331,0.002359826,0.003741661],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0008017004,"about_ca_system_score_gemma":0.0005313313,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001804801,"about_ca_topic_score_gemma":0.004812031,"domain_scores_codex":[0.9993255,0.00008886421,0.00009624744,0.0002070816,0.0001890471,0.00009319855],"domain_scores_gemma":[0.9997395,0.00003920841,0.0000528343,0.00004935061,0.00009674385,0.0000223509],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004050374,0.00003012493,0.0001139548,0.0001210113,0.00001062975,0.00004137633,0.00003478745,0.0000476644,0.9973662,0.000281398,0.0003088183,0.001603573],"study_design_scores_gemma":[0.00002348462,0.0001631744,0.006618981,0.00005166911,0.00004959137,0.0003558015,0.00009955207,0.001655594,0.9667526,0.0002826284,0.02392183,0.0000251576],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.3652786,0.01031946,0.5487299,0.002354545,0.001359081,0.003403086,0.03676022,0.007429881,0.02436521],"genre_scores_gemma":[0.310216,0.02071653,0.5815872,0.001196112,0.0002780824,0.004592189,0.03657989,0.001989328,0.04284473],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.003762549,"threshold_uncertainty_score":0.01258695,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01728544017408916,"score_gpt":0.2899243326089793,"score_spread":0.2726388924348901,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}