{"id":"W4389799374","doi":"10.1101/pdb.prot108283","title":"Membrane Protein Fractionation and Analysis through Western Blot in<i>Aedes aegypti</i>Malpighian Tubules","year":2023,"lang":"en","type":"article","venue":"Cold Spring Harbor Protocols","topic":"Insect Resistance and Genetics","field":"Biochemistry, Genetics and Molecular Biology","cited_by":3,"is_retracted":false,"has_abstract":true,"ca_institutions":"York University","funders":"","keywords":"Western blot; Fractionation; Membrane protein; Cell fractionation; Blot; Biology; Cytosol; Biochemistry; Protein purification; Bacterial outer membrane; Chemistry; Membrane; Chromatography","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002332934,0.0001581038,0.0001926406,0.0001425938,0.00009722053,0.00007348025,0.000142009,0.0001417284,0.000009765203],"category_scores_gemma":[0.00005389929,0.0001639388,0.00007511528,0.000510237,0.00005076563,0.00001308593,0.0000912182,0.00009850783,0.00001555068],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001950286,"about_ca_system_score_gemma":0.00005277858,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00004849581,"about_ca_topic_score_gemma":0.0003324176,"domain_scores_codex":[0.9988682,0.00005490421,0.0002406953,0.0003968196,0.0001732836,0.000266156],"domain_scores_gemma":[0.9994497,0.00001215562,0.00008956229,0.0003314098,0.00006989484,0.00004722821],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00008232546,0.00005159011,0.04779879,0.0001134666,0.0001170864,0.000009329462,0.0000390214,0.0002845252,0.9507606,0.0006030863,0.00003344379,0.0001067259],"study_design_scores_gemma":[0.0006185439,0.0001424143,0.2172074,0.00007397895,0.0000397657,3.377531e-7,0.00001627512,0.0001828466,0.741033,0.00004041681,0.04039942,0.0002456731],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9844368,0.00009193948,0.0005781159,0.0001872273,0.00001952409,0.01444645,0.0000113906,0.00004722194,0.0001813177],"genre_scores_gemma":[0.966552,0.00005957014,0.0009787795,0.0001143683,0.0001187369,0.03157881,0.00001437299,0.00002728862,0.0005561442],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.2097276,"threshold_uncertainty_score":0.6685232,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01728544017408916,"score_gpt":0.2899243326089793,"score_spread":0.2726388924348901,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}