{"id":"W4389800384","doi":"10.1101/pdb.prot108280","title":"Examining Cell-Specific Localization in<i>Aedes aegypti</i>Tissues with Fluorescence In Situ Hybridization","year":2023,"lang":"en","type":"article","venue":"Cold Spring Harbor Protocols","topic":"RNA and protein synthesis mechanisms","field":"Biochemistry, Genetics and Molecular Biology","cited_by":3,"is_retracted":false,"has_abstract":true,"ca_institutions":"York University","funders":"","keywords":"In situ hybridization; Aedes aegypti; Biology; Digoxigenin; RNA; Fluorescence in situ hybridization; In situ; Hybridization probe; Molecular biology; DNA; Riboprobe; Fluorescence microscope; Computational biology; Fluorescence; Cell biology; Gene expression; Gene; Genetics; Chemistry; Chromosome","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004113,0.0001707307,0.0001665622,0.0001444355,0.0000641482,0.00006838339,0.0002031915,0.000116493,0.000008301731],"category_scores_gemma":[0.00005490015,0.000170772,0.00002180538,0.0004483103,0.00003468408,0.00001510871,0.00008107565,0.00008032166,0.00002583903],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00003165775,"about_ca_system_score_gemma":0.00005657995,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001397567,"about_ca_topic_score_gemma":0.00002395297,"domain_scores_codex":[0.9986984,0.00009857227,0.0002620471,0.0004512473,0.0001828153,0.0003069127],"domain_scores_gemma":[0.9994377,0.00001874177,0.0000963769,0.0003429875,0.00005200742,0.00005218622],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00009294296,0.00004521888,0.01346557,0.00008627613,0.000003269431,0.00002575164,0.00004505192,0.001169846,0.9840656,0.00044129,0.00008012253,0.0004790622],"study_design_scores_gemma":[0.000643249,0.0002232552,0.009564186,0.0003574534,0.000001863923,3.945161e-7,0.00003382723,0.000305003,0.9733618,0.00001521023,0.01526059,0.0002331399],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9399915,0.0002421771,0.02114831,0.0000553748,0.00006484782,0.03782418,0.000003956063,0.0001295213,0.0005400813],"genre_scores_gemma":[0.9662155,0.00007929844,0.002054838,0.00003564979,0.00008218745,0.03136665,0.000007565909,0.00004494875,0.000113378],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.02622393,"threshold_uncertainty_score":0.6963881,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02523601924143983,"score_gpt":0.2544252909783531,"score_spread":0.2291892717369133,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}