{"id":"W4389818663","doi":"10.1002/rcm.9672","title":"Development and application of a multiple reaction monitoring method for the simultaneous quantification of sodium channels Na <sub>v</sub> 1.1, Na <sub>v</sub> 1.2, and Na <sub>v</sub> 1.6 in solubilized membrane proteins from stable HEK293 cell lines, rodents, and human brain tissues","year":2023,"lang":"en","type":"article","venue":"Rapid Communications in Mass Spectrometry","topic":"Ion channel regulation and function","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"Xenon Pharmaceuticals (Canada)","funders":"","keywords":"Chemistry; HEK 293 cells; Sodium; Transfection; Electrospray ionization; Sodium channel; Membrane; Molecular biology; Mass spectrometry; Chromatography; Biochemistry; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.002468698,0.001479659,0.0007023722,0.001037966,0.0005098592,0.0007677023,0.001193328,0.001684161,0.000979283],"category_scores_gemma":[0.001864761,0.0008652465,0.0007377987,0.0006041575,0.0007319936,0.0006969204,0.0005926874,0.001730263,0.001087527],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005484578,"about_ca_system_score_gemma":0.001256138,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0006548617,"about_ca_topic_score_gemma":0.001325408,"domain_scores_codex":[0.9973599,0.0005408461,0.000142603,0.001069103,0.0007790878,0.0001084033],"domain_scores_gemma":[0.9988952,0.0003316179,0.0002334145,0.0001177817,0.0003131803,0.0001088455],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004245186,0.0000360453,0.0001926416,0.00005942423,0.00001315122,0.00002912858,0.00002497121,0.00009858218,0.9928246,0.0001457223,0.00008162149,0.006451704],"study_design_scores_gemma":[0.00002677821,0.0004270277,0.001389235,0.00001570674,0.00003890169,0.0006154239,0.00003017948,0.006221171,0.9877859,0.0001788947,0.003236347,0.00003455922],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"empirical","genre_scores_codex":[0.1019715,0.001693989,0.8906531,0.0003414006,0.0002150511,0.000714973,0.0005031983,0.001805645,0.002101134],"genre_scores_gemma":[0.1237435,0.001465571,0.8684899,0.0004181595,0.00005438696,0.001764458,0.0005974889,0.0001107357,0.003355757],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.002468698,"threshold_uncertainty_score":0.01305592,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02818002314228517,"score_gpt":0.2949075925751422,"score_spread":0.266727569432857,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}