{"id":"W4389935020","doi":"10.1021/acssynbio.3c00399","title":"An Expanded Genetic Toolbox to Accelerate the Creation of <i>Acholeplasma laidlawii</i> Driven by Synthetic Genomes","year":2023,"lang":"en","type":"article","venue":"ACS Synthetic Biology","topic":"Microbial infections and disease research","field":"Immunology and Microbiology","cited_by":2,"is_retracted":false,"has_abstract":true,"ca_institutions":"Western University","funders":"Natural Sciences and Engineering Research Council of Canada; Universiteit van Amsterdam; Government of Canada","keywords":"Mollicutes; Genome; Biology; Electroporation; Transposable element; Synthetic biology; Genetics; Mobile genetic elements; Bacterial genome size; Gene; Computational biology; Bacteria","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000799717,0.0009112626,0.0005156654,0.0007589296,0.0003078243,0.0007524007,0.0008355217,0.0005870092,0.002365064],"category_scores_gemma":[0.0005642844,0.0004525022,0.0006961192,0.0004002215,0.0003511556,0.0005234041,0.001037287,0.001563029,0.001588138],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003890375,"about_ca_system_score_gemma":0.0004810791,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004927607,"about_ca_topic_score_gemma":0.0007046165,"domain_scores_codex":[0.9996027,0.00006730833,0.0000503599,0.00007660696,0.0001525977,0.00005037047],"domain_scores_gemma":[0.999635,0.00008979557,0.00009603277,0.00006558366,0.00003502148,0.00007851313],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005026456,0.000057869,0.0001466631,0.0001006817,0.000009611547,0.0001100315,0.00004086406,0.0005035325,0.9926981,0.0008487724,0.0001773061,0.00525621],"study_design_scores_gemma":[0.00005673811,0.0003101428,0.001213718,0.00004670846,0.00003744628,0.0005818744,0.00004861833,0.005633396,0.9647579,0.0004978037,0.02678002,0.00003572291],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.5720736,0.001520735,0.4001228,0.0009907708,0.0004037652,0.001017096,0.004766804,0.009737753,0.009366636],"genre_scores_gemma":[0.5892582,0.002314674,0.3900494,0.0002269292,0.00006119915,0.0007365778,0.007379201,0.002038494,0.007935375],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002365064,"threshold_uncertainty_score":0.007911861,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01908623526857994,"score_gpt":0.2959359465124468,"score_spread":0.2768497112438669,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}