{"id":"W4390348141","doi":"10.1002/cpz1.965","title":"Production of CRISPR‐Cas9 Transgenic Cell Lines for Knocksideways Studies","year":2023,"lang":"en","type":"article","venue":"Current Protocols","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":5,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University","funders":"National Institute of General Medical Sciences; University of Washington; National Institutes of Health; National Science Foundation","keywords":"CRISPR; Context (archaeology); Synthetic biology; Computational biology; Green fluorescent protein; Transgene; Genome editing; Cas9; Biology; Computer science; Gene; Genetics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002040185,0.0001238522,0.0001560454,0.00005330993,0.00004499655,0.00000649788,0.0001098419,0.00005762564,0.000002322426],"category_scores_gemma":[0.0001224375,0.0001132357,0.0001141981,0.0001362208,0.00003491903,0.000002661295,0.00004184528,0.00004782973,0.000004742477],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000005298835,"about_ca_system_score_gemma":0.00003614044,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":2.968038e-7,"about_ca_topic_score_gemma":0.000002492547,"domain_scores_codex":[0.9992012,0.00001491739,0.0002351694,0.0002673416,0.00009242215,0.0001889853],"domain_scores_gemma":[0.9994848,0.00001155676,0.00005837523,0.0002390725,0.0001735072,0.00003269982],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00006879215,0.00007817797,0.000286396,0.001631021,0.00003780283,1.15618e-7,0.0001827495,0.001014706,0.9673753,0.00001553784,0.01519325,0.01411609],"study_design_scores_gemma":[0.0004043424,0.0002374749,0.000219658,0.00008258122,0.00001633118,6.310383e-7,0.00009237039,0.00005840353,0.8667375,0.00009375549,0.1319405,0.0001164009],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.7572446,0.01212391,0.0531047,0.0004595736,0.003047751,0.1734817,0.0001300509,0.0002428355,0.0001648473],"genre_scores_gemma":[0.7708696,0.001296842,0.001567186,0.00001376298,0.001620244,0.2226761,0.0001884549,0.00007560175,0.001692239],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1167472,"threshold_uncertainty_score":0.4617618,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.08593432879648592,"score_gpt":0.4544464393445899,"score_spread":0.368512110548104,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}