{"id":"W4390538633","doi":"10.12688/f1000research.145146.1","title":"A guide to selecting high-performing antibodies for Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform (PPP2R5D) for use in Western Blot, immunoprecipitation and immunofluorescence","year":2024,"lang":"en","type":"preprint","venue":"F1000Research","topic":"Ubiquitin and proteasome pathways","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University; Montreal Neurological Institute and Hospital","funders":"Canadian Institutes of Health Research; Genentech; Government of Canada; Ontario Genomics Institute; European Federation of Pharmaceutical Industries and Associations; Merck KGaA; Mitacs; Ontario Genomics; Genome Canada; Simons Foundation Autism Research Initiative; Bayer; Pfizer; Bristol-Myers Squibb","keywords":"Gene isoform; Immunoprecipitation; Western blot; Phosphatase; Biology; Protein phosphatase 2; Serine; Antibody; Protein subunit; Phosphorylation; Immunofluorescence; Molecular biology; Cell biology; Gene; Genetics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.001910604,0.0005001627,0.0004771989,0.000406634,0.0002878242,0.0004164434,0.0006306938,0.0005029818,0.00001123873],"category_scores_gemma":[0.0008844307,0.0005213183,0.0001913982,0.0002513164,0.0001331312,0.00002945317,0.002216201,0.0006902677,0.00001018279],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001553034,"about_ca_system_score_gemma":0.0005580774,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002447233,"about_ca_topic_score_gemma":0.001697645,"domain_scores_codex":[0.9966913,0.0001246779,0.0006964901,0.001179938,0.0003767801,0.0009308333],"domain_scores_gemma":[0.9980523,0.0001797943,0.0001538572,0.0008268091,0.0006011939,0.0001860603],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.002479469,0.000118428,0.004072988,0.004111912,0.0003240426,0.00002163178,0.0009575149,0.0002932574,0.9460137,0.0004642201,0.003427172,0.03771561],"study_design_scores_gemma":[0.001392382,0.001024746,0.00300754,0.001557393,0.00005214176,0.00001844624,0.0002874146,0.002068122,0.9691315,0.0009876019,0.01966191,0.0008108072],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9836705,0.007830284,0.001618977,0.0002994364,0.0002740683,0.0048642,0.001339918,0.00005971825,0.00004288036],"genre_scores_gemma":[0.9555935,0.0008016379,0.03029728,0.0001082352,0.0006051125,0.004113575,0.002861684,0.000223805,0.005395222],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0369048,"threshold_uncertainty_score":0.9997239,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03147382949848877,"score_gpt":0.3309182737983771,"score_spread":0.2994444442998884,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}