{"id":"W4390618247","doi":"10.1093/nar/gkad1247","title":"Intein-based thermoregulated meganucleases for containment of genetic material","year":2024,"lang":"en","type":"article","venue":"Nucleic Acids Research","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":12,"is_retracted":false,"has_abstract":true,"ca_institutions":"Western University","funders":"Natural Sciences and Engineering Research Council of Canada; Canadian Institutes of Health Research","keywords":"Intein; Biology; Plasmid; Homing endonuclease; Escherichia coli; Endonuclease; Synthetic biology; Mutagenesis; DNA; Genetics; Computational biology; Gene; Mutation; RNA splicing","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002267883,0.0004006109,0.0002396768,0.0002338503,0.0001161214,0.0003468359,0.0003109711,0.0002785746,0.0005671843],"category_scores_gemma":[0.000294949,0.0001923335,0.0002732015,0.0001880305,0.0002788098,0.000294505,0.0004720373,0.0008883761,0.0003716052],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002412045,"about_ca_system_score_gemma":0.000174653,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001674799,"about_ca_topic_score_gemma":0.0004782927,"domain_scores_codex":[0.9997604,0.00004495764,0.000028548,0.00004437526,0.00009226523,0.00002937589],"domain_scores_gemma":[0.9997789,0.00005019092,0.00008648512,0.0000448214,0.0000159145,0.00002353189],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001834554,0.000007647624,0.00009495451,0.00002055283,0.000003251238,0.00001450018,0.000007832948,0.00007302256,0.9981212,0.0001628179,0.00001195209,0.001463944],"study_design_scores_gemma":[0.000001770219,0.00003408275,0.0005341378,0.000003197998,0.000005336816,0.0001115981,0.000004868889,0.0005150965,0.9975067,0.00004289003,0.001237726,0.000002602219],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8603231,0.002220983,0.1346667,0.0002155755,0.00006850228,0.0001043038,0.0003890253,0.0004365257,0.001575339],"genre_scores_gemma":[0.9191787,0.00139592,0.07522616,0.000070568,0.0000113989,0.00006487514,0.0006554524,0.000124318,0.003272524],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0005671843,"threshold_uncertainty_score":0.001897454,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02108437209951899,"score_gpt":0.3628113965056464,"score_spread":0.3417270244061275,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}