{"id":"W4390618247","doi":"10.1093/nar/gkad1247","title":"Intein-based thermoregulated meganucleases for containment of genetic material","year":2024,"lang":"en","type":"article","venue":"Nucleic Acids Research","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":12,"is_retracted":false,"has_abstract":true,"ca_institutions":"Western University","funders":"Natural Sciences and Engineering Research Council of Canada; Canadian Institutes of Health Research","keywords":"Intein; Biology; Plasmid; Homing endonuclease; Escherichia coli; Endonuclease; Synthetic biology; Mutagenesis; DNA; Genetics; Computational biology; Gene; Mutation; RNA splicing","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003584145,0.0001128357,0.000124799,0.0001085511,0.00005162758,0.00004663028,0.0002164391,0.0001145984,0.0001694338],"category_scores_gemma":[0.00008428546,0.0001013694,0.00009768569,0.000132529,0.0001338646,0.000001676057,0.00008886892,0.00008359036,0.000009030471],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0000254472,"about_ca_system_score_gemma":0.000108069,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001923312,"about_ca_topic_score_gemma":0.000004767123,"domain_scores_codex":[0.9988927,0.00005318235,0.0001987559,0.0002999342,0.0002400603,0.0003153182],"domain_scores_gemma":[0.9994241,0.00004506038,0.0000139762,0.0003030313,0.0001404225,0.00007343205],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002320388,0.00003676948,0.00009667614,0.0001990578,0.00005888658,0.000004970946,0.00004502246,0.0003795736,0.9854215,0.0001536459,0.002467896,0.01090401],"study_design_scores_gemma":[0.0003981261,0.0007430158,0.001808732,0.00006682158,0.00001458823,0.000004355003,0.00007963058,0.007500013,0.9532548,0.0001311863,0.03587031,0.0001284253],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9861854,0.001319586,0.01128496,0.0001524608,0.0002024682,0.0004275692,0.00008768614,0.00002715757,0.0003126666],"genre_scores_gemma":[0.9974329,0.00004783171,0.001752046,0.00002217633,0.0002379046,0.00006660527,0.00006887436,0.00004745974,0.000324182],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.03340241,"threshold_uncertainty_score":0.4133725,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02108437209951899,"score_gpt":0.3628113965056464,"score_spread":0.3417270244061275,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}