{"id":"W4390631004","doi":"10.3791/66471","title":"Characterizing Multidrug Efflux Systems in <em>Acinetobacter baumannii</em> Using an Efflux-Deficient Bacterial Strain and a Single-Copy Gene Expression System","year":2024,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"Antibiotic Resistance in Bacteria","field":"Biochemistry, Genetics and Molecular Biology","cited_by":1,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Manitoba","funders":"","keywords":"Efflux; Acinetobacter baumannii; Biology; Plasmid; Gene; Microbiology; Operon; Bacterial genetics; Multiple drug resistance; Genetics; Antibiotic resistance; Gene expression; Drug resistance; Antibiotics; Bacteria; Pseudomonas aeruginosa; Escherichia coli","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003542665,0.0004236458,0.0003765585,0.0003014921,0.0002063993,0.0004223396,0.0002878368,0.0003547098,0.0003930138],"category_scores_gemma":[0.0003915659,0.0001769839,0.0002034289,0.0004100767,0.0002337334,0.000308108,0.0003025979,0.0005648319,0.0003836252],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005224767,"about_ca_system_score_gemma":0.000314488,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002283438,"about_ca_topic_score_gemma":0.002609048,"domain_scores_codex":[0.9996696,0.00006075456,0.00005388612,0.00005560444,0.0001076067,0.00005263182],"domain_scores_gemma":[0.9998049,0.00003602521,0.00005892613,0.00002787176,0.00004440729,0.00002791046],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001444598,0.00002371495,0.0001252105,0.00001635739,0.000001613452,0.00001213002,0.00000905393,0.00006184642,0.9991295,0.00003540186,0.00001632605,0.0005544628],"study_design_scores_gemma":[0.00000634607,0.000173527,0.002657932,0.000005185131,0.000009145725,0.0001322884,0.00004909289,0.001770619,0.9937715,0.00004483168,0.001371659,0.000007865663],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9845026,0.000544607,0.01209207,0.0002102258,0.00002206216,0.0001004895,0.001320157,0.0001611048,0.001046659],"genre_scores_gemma":[0.9607942,0.0008490285,0.03139096,0.0001229277,0.0000114423,0.0001585029,0.003944398,0.0001256443,0.002602838],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002283438,"threshold_uncertainty_score":0.004540324,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02808951033919603,"score_gpt":0.3463202244642414,"score_spread":0.3182307141250453,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}