{"id":"W4391023230","doi":"10.1038/s41596-023-00930-8","title":"Production and use of antigen tetramers to study antigen-specific B cells","year":2024,"lang":"en","type":"review","venue":"Nature Protocols","topic":"T-cell and B-cell Immunology","field":"Immunology and Microbiology","cited_by":23,"is_retracted":false,"has_abstract":false,"ca_institutions":"McMaster University; McMaster University Medical Centre","funders":"National Institute of Allergy and Infectious Diseases; U.S. Department of Health and Human Services","keywords":"Antigen; Tetramer; B-cell receptor; Biotinylation; Alexa Fluor; Flow cytometry; Antibody; Streptamer; Streptavidin; Antigen-presenting cell; Molecular biology; B cell; Biology; Chemistry; Immune system; T cell; Cell biology; Immunology; Fluorescence; Biochemistry; Biotin","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001501334,0.002106927,0.001213079,0.00195448,0.0003265892,0.001217018,0.001900595,0.001472882,0.003224085],"category_scores_gemma":[0.0007531084,0.000782004,0.0005613466,0.001481712,0.001150494,0.00200333,0.001341585,0.0038309,0.006464108],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.001265107,"about_ca_system_score_gemma":0.001073016,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007664184,"about_ca_topic_score_gemma":0.0009776889,"domain_scores_codex":[0.999642,0.0000495739,0.00003095863,0.00006358923,0.0001673258,0.00004653423],"domain_scores_gemma":[0.9996847,0.0001230317,0.00003455621,0.00002897102,0.00008756068,0.00004113973],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"design_other","study_design_gemma":"not_applicable","study_design_scores_codex":[0.0001285745,0.0001477556,0.00009422636,0.00654675,0.00004991208,0.000258088,0.00005257005,0.0009119604,0.05563115,0.0221542,0.05897532,0.8550494],"study_design_scores_gemma":[0.00003920637,0.00009921041,0.0002290363,0.0005498239,0.00004102856,0.0004356745,0.00001451452,0.0002924367,0.02284507,0.004422972,0.9710039,0.00002714182],"study_design_candidate":"not_applicable","study_design_consensus":null,"genre_codex":"review","genre_gemma":"methods","genre_scores_codex":[0.0008799051,0.9683954,0.01474817,0.001420496,0.004319604,0.0001263954,0.0002185222,0.0001566899,0.009734765],"genre_scores_gemma":[0.002924875,0.9742585,0.008726148,0.001064072,0.001023801,0.0002275084,0.0004166903,0.0000534681,0.01130498],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.003224085,"threshold_uncertainty_score":0.01078558,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.04896592852317282,"score_gpt":0.3464749980875498,"score_spread":0.297509069564377,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}