{"id":"W4391163995","doi":"10.1002/cbic.202300773","title":"Investigating Protein Binding with the Isothermal Ligand‐induced Resolubilization Assay","year":2024,"lang":"en","type":"article","venue":"ChemBioChem","topic":"Biochemical and Molecular Research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"Natural Sciences and Engineering Research Council of Canada; Canadian Institutes of Health Research; Michael Smith Health Research BC","keywords":"Ligand (biochemistry); Solubility; Chemistry; Dihydrofolate reductase; Protein ligand; Biochemistry; Biophysics; Biology; Enzyme; Receptor","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001256359,0.001105237,0.0008343143,0.0006579875,0.000446708,0.0006977065,0.00112141,0.0008904978,0.001854699],"category_scores_gemma":[0.001453679,0.0004718254,0.0006337618,0.0006162376,0.0006082594,0.0005842606,0.000793334,0.002106544,0.001380854],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0008203698,"about_ca_system_score_gemma":0.0003943085,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007234036,"about_ca_topic_score_gemma":0.001073948,"domain_scores_codex":[0.9976065,0.0006098684,0.0001139083,0.0004775135,0.0009027345,0.0002893941],"domain_scores_gemma":[0.9985265,0.0005772408,0.0003640995,0.0001570252,0.0002575033,0.00011761],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000311277,0.00002961797,0.00009928343,0.00003230487,0.000007771705,0.00001044005,0.00002593582,0.0001117776,0.9985471,0.00006413901,0.00004432864,0.0009962432],"study_design_scores_gemma":[0.000003233697,0.00008901992,0.0004155471,0.000002164879,0.000005639785,0.00003740496,0.00001198286,0.002162057,0.9966965,0.00002758116,0.0005402826,0.000008644541],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.6637824,0.001916837,0.3266974,0.0003693659,0.0001095053,0.0002902962,0.0009056088,0.002113555,0.003815069],"genre_scores_gemma":[0.8309436,0.001995318,0.1539904,0.000453586,0.00006743569,0.0006467769,0.001834083,0.0003602376,0.009708679],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001854699,"threshold_uncertainty_score":0.006644368,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01743410806621441,"score_gpt":0.2663404404648648,"score_spread":0.2489063323986503,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}