{"id":"W4391226330","doi":"10.1101/2024.01.24.577072","title":"Development of a qPCR assay and a LAMP assay for <i>Verticillium longisporum</i> detection and a triplex qPCR assay for simultaneous detection of <i>V. longisporum</i> , <i>Leptosphaeria biglobosa</i> and <i>L. maculans</i> from canola samples","year":2024,"lang":"en","type":"preprint","venue":"bioRxiv (Cold Spring Harbor Laboratory)","topic":"Plant Pathogens and Fungal Diseases","field":"Biochemistry, Genetics and Molecular Biology","cited_by":1,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Manitoba; Agriculture Food and Rural Development; University of Saskatchewan; Alberta Health","funders":"","keywords":"Blackleg; Canola; Leptosphaeria maculans; Biology; Verticillium; Luciferases; Loop-mediated isothermal amplification; Brassica; Gene; Botany; Genetics; DNA; Luciferase","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":true,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001747391,0.001280281,0.001023433,0.00130119,0.0004690237,0.0007375533,0.0007766578,0.0009684727,0.001385315],"category_scores_gemma":[0.001450604,0.0008257795,0.001093414,0.0006810023,0.0006300303,0.0004969699,0.0007616502,0.001080509,0.001153055],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0008976575,"about_ca_system_score_gemma":0.001736403,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.004438938,"about_ca_topic_score_gemma":0.009430492,"domain_scores_codex":[0.9975886,0.0003411543,0.0001836428,0.0007482399,0.0008428438,0.0002955766],"domain_scores_gemma":[0.9983904,0.0004500945,0.0002622778,0.0001561485,0.0006142976,0.0001268336],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002655036,0.00003973294,0.0007744047,0.00005127448,0.000006912166,0.00001446749,0.0000447625,0.0001990675,0.9962035,0.00007639256,0.00008348862,0.002479422],"study_design_scores_gemma":[0.00001311938,0.0003147978,0.004573909,0.00002476331,0.00004836811,0.00009532993,0.00009800491,0.005957868,0.9863202,0.00007489564,0.002444263,0.00003444712],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.5183565,0.0007888787,0.4661619,0.0004064412,0.0002772532,0.001648433,0.004378691,0.00326779,0.004714068],"genre_scores_gemma":[0.4824916,0.0008249584,0.489055,0.0005917545,0.00006821801,0.003323748,0.007347285,0.0002809528,0.01601651],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.004438938,"threshold_uncertainty_score":0.009241164,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01132275950357495,"score_gpt":0.2138945511470653,"score_spread":0.2025717916434904,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}