{"id":"W4391521971","doi":"10.1101/2024.02.03.24302274","title":"Underestimation of SARS-CoV-2 in wastewater due to single or double mutations in the N1 qPCR probe binding region","year":2024,"lang":"en","type":"preprint","venue":"medRxiv","topic":"SARS-CoV-2 detection and testing","field":"Medicine","cited_by":1,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Mutation; Wastewater; Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2); Allele; Coronavirus disease 2019 (COVID-19); Biology; Molecular biology; Gene; Virology; Genetics; Medicine; Internal medicine; Environmental science","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0009690357,0.000685475,0.0004888137,0.0005420232,0.0003738111,0.0008624911,0.0003080833,0.0007506358,0.001133496],"category_scores_gemma":[0.001649003,0.0003584103,0.000419275,0.0004626997,0.0004563755,0.0004142172,0.0005167415,0.0004933075,0.0005581266],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005405621,"about_ca_system_score_gemma":0.000361868,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.003312585,"about_ca_topic_score_gemma":0.00448753,"domain_scores_codex":[0.9980592,0.0002604806,0.0001260193,0.0005877846,0.0007567052,0.0002097532],"domain_scores_gemma":[0.999294,0.0001912808,0.0001960979,0.00005163456,0.0002323224,0.00003461803],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001780269,0.00003671964,0.02952342,0.0001208205,0.00002251073,0.00005568706,0.0001310676,0.0002888854,0.9644408,0.00003691412,0.000121071,0.005044049],"study_design_scores_gemma":[0.000007116382,0.0004242648,0.1366093,0.00004465415,0.00006782271,0.0004085673,0.0004911032,0.00633905,0.8524547,0.0001842477,0.00293688,0.00003241059],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9812436,0.0009900316,0.0145489,0.0001206568,0.00006302312,0.00006301098,0.001115781,0.0002283068,0.001626658],"genre_scores_gemma":[0.9884428,0.0003217889,0.00847202,0.0001578867,0.000009774473,0.00008479355,0.0009429146,0.00006098662,0.001506969],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.003312585,"threshold_uncertainty_score":0.006586611,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.1665259648012433,"score_gpt":0.3621440616580242,"score_spread":0.1956180968567809,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}