{"id":"W4391575341","doi":"10.1038/s41587-023-02106-x","title":"Efficient prime editing in two-cell mouse embryos using PEmbryo","year":2024,"lang":"en","type":"article","venue":"Nature Biotechnology","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":22,"is_retracted":false,"has_abstract":true,"ca_institutions":"Hospital for Sick Children; University of Toronto","funders":"Eunice Kennedy Shriver National Institute of Child Health and Human Development; Rutgers Cancer Institute of New Jersey; National Institute of Biomedical Imaging and Bioengineering; National Institute of Allergy and Infectious Diseases; National Institute of General Medical Sciences; National Center for Advancing Translational Sciences; National Human Genome Research Institute; Searle Scholars Program; National Institute of Diabetes and Digestive and Kidney Diseases; Princeton University; National Cancer Institute; National Institutes of Health; National Science Foundation","keywords":"Indel; Biology; Embryo; Genetics; Computational biology; Genome; Genome editing; Phenotype; Prime (order theory); DNA; Cell biology; Molecular biology; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001571102,0.0001835957,0.0001448924,0.0002475888,0.00003574353,0.0000233738,0.0002107055,0.0008582549,0.00000899215],"category_scores_gemma":[0.00006891357,0.0001824159,0.00007194994,0.0003066896,0.00006043513,0.00000127918,0.0001735052,0.0007654534,0.00001183416],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00003853979,"about_ca_system_score_gemma":0.00005652312,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001516564,"about_ca_topic_score_gemma":0.0000259537,"domain_scores_codex":[0.9988523,0.00001746097,0.0001882659,0.0004909463,0.00009660013,0.0003544538],"domain_scores_gemma":[0.9995618,0.00001051373,0.00002592239,0.0003390379,0.0000231194,0.00003958493],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000007784321,0.00002882431,0.0000673736,0.00004276316,0.00001292437,0.00002896662,0.00001793129,0.007027276,0.9905623,0.0003082527,0.0001923426,0.001703294],"study_design_scores_gemma":[0.0002932972,0.00005190932,0.00003959536,0.00003416319,0.00001081355,0.00005672314,0.00005849302,0.03052808,0.9565421,0.00001761803,0.0121726,0.0001945935],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9727597,0.01393769,0.01145225,0.0003161833,0.0008284018,0.0001676327,0.00001184933,0.0001376961,0.0003885661],"genre_scores_gemma":[0.9960576,0.00007476368,0.003020324,0.00007592399,0.0003898527,0.000007278436,0.00002171675,0.00003891088,0.0003135759],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.03402015,"threshold_uncertainty_score":0.7438703,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.003786184979788,"score_gpt":0.2933683498851544,"score_spread":0.2895821649053664,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}