{"id":"W4391886444","doi":"10.1093/biomethods/bpae009","title":"A methodological primer of extracellular vesicles isolation and characterization via different techniques","year":2024,"lang":"en","type":"article","venue":"Biology Methods and Protocols","topic":"Extracellular vesicles in disease","field":"Biochemistry, Genetics and Molecular Biology","cited_by":35,"is_retracted":false,"has_abstract":true,"ca_institutions":"Western University","funders":"Natural Sciences and Engineering Research Council of Canada; Canadian Institutes of Health Research; Universities Space Research Association","keywords":"Extracellular vesicles; Isolation (microbiology); Characterization (materials science); Primer (cosmetics); Vesicle; Chemistry; Biology; Nanotechnology; Biochemistry; Cell biology; Materials science; Bioinformatics; Organic chemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.005187225,0.001566943,0.001227796,0.003096211,0.001578858,0.00293483,0.001615502,0.00151765,0.003009957],"category_scores_gemma":[0.003467036,0.001191893,0.001383244,0.002132135,0.001794014,0.002756556,0.002404321,0.005353515,0.005115981],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0007435845,"about_ca_system_score_gemma":0.001618664,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005320523,"about_ca_topic_score_gemma":0.0005215235,"domain_scores_codex":[0.9963167,0.0009011469,0.0007759132,0.00074757,0.00108821,0.0001705036],"domain_scores_gemma":[0.9977753,0.0005328566,0.000245303,0.0006276882,0.0007163394,0.0001027],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"not_applicable","study_design_scores_codex":[0.0002384838,0.000325704,0.001216152,0.004993359,0.0001488571,0.0007767504,0.001158893,0.001184879,0.7105734,0.06592208,0.0113311,0.2021303],"study_design_scores_gemma":[0.00002218368,0.0004114441,0.001730279,0.0007985577,0.00009100218,0.002134669,0.0002575835,0.001633984,0.3462258,0.01430933,0.6322311,0.0001540315],"study_design_candidate":"not_applicable","study_design_consensus":null,"genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.008398738,0.03942958,0.9345796,0.003218858,0.003206897,0.001468837,0.001180264,0.0009842452,0.007532965],"genre_scores_gemma":[0.01983758,0.06478232,0.8941236,0.002458687,0.001071869,0.003497159,0.003120406,0.0007371336,0.01037145],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.005187225,"threshold_uncertainty_score":0.02743304,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.07993833077970179,"score_gpt":0.435639324230338,"score_spread":0.3557009934506362,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}