{"id":"W4392060233","doi":"10.21769/bioprotoc.4951","title":"Suppression of Host Trafficking to Lysosomes: Fluorescence Microscopy-Based DQ-Red BSA Analysis","year":2024,"lang":"en","type":"article","venue":"BIO-PROTOCOL","topic":"Bacterial biofilms and quorum sensing","field":"Biochemistry, Genetics and Molecular Biology","cited_by":1,"is_retracted":false,"has_abstract":true,"ca_institutions":"Institute of Infection and Immunity; University of Ottawa","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Salmonella enterica; Virulence; Biology; Salmonella; Pathogen; Microbiology; Intracellular parasite; Phenotype; Intracellular; Cell biology; Fluorescence microscope; Host–pathogen interaction; Bacteria; Gene; Genetics; Fluorescence","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004960433,0.0004882072,0.0002874424,0.0004139003,0.0002731817,0.0003988229,0.0003872906,0.0004933649,0.001400158],"category_scores_gemma":[0.0001939269,0.0002310269,0.000405938,0.0002420278,0.000282526,0.0002508194,0.0003274981,0.000722468,0.0008176098],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006219922,"about_ca_system_score_gemma":0.0003069083,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00183028,"about_ca_topic_score_gemma":0.001981307,"domain_scores_codex":[0.999773,0.00004839992,0.00002047092,0.0000586379,0.00004810018,0.00005133654],"domain_scores_gemma":[0.9997761,0.00004973456,0.00005214215,0.00003812306,0.00005248149,0.0000315564],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003436241,0.00001273651,0.00006151121,0.00002196218,0.000002349707,0.00002009204,0.00001262449,0.0000462574,0.9992944,0.0001029735,0.00002543428,0.0003652369],"study_design_scores_gemma":[0.000005387074,0.00004787665,0.00132836,0.000004954009,0.000005668036,0.0001289776,0.00001745313,0.001407583,0.9961903,0.00003743131,0.0008214604,0.000004551794],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.8898135,0.001133631,0.1021835,0.0003855557,0.00007492279,0.0002151916,0.001346208,0.0007220036,0.004125545],"genre_scores_gemma":[0.9319562,0.001313521,0.05734447,0.0001373225,0.00001133947,0.0002463854,0.001385134,0.0003245475,0.007281004],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.00183028,"threshold_uncertainty_score":0.004683971,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01204865817108594,"score_gpt":0.3081105581292291,"score_spread":0.2960618999581432,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}