{"id":"W4392060233","doi":"10.21769/bioprotoc.4951","title":"Suppression of Host Trafficking to Lysosomes: Fluorescence Microscopy-Based DQ-Red BSA Analysis","year":2024,"lang":"en","type":"article","venue":"BIO-PROTOCOL","topic":"Bacterial biofilms and quorum sensing","field":"Biochemistry, Genetics and Molecular Biology","cited_by":1,"is_retracted":false,"has_abstract":true,"ca_institutions":"Institute of Infection and Immunity; University of Ottawa","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Salmonella enterica; Virulence; Biology; Salmonella; Pathogen; Microbiology; Intracellular parasite; Phenotype; Intracellular; Cell biology; Fluorescence microscope; Host–pathogen interaction; Bacteria; Gene; Genetics; Fluorescence","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001795291,0.0002285072,0.0002735006,0.0002007702,0.00007600334,0.00009417432,0.0002171303,0.0002098747,0.00009305594],"category_scores_gemma":[0.00004189874,0.0001910821,0.0002631517,0.0006122131,0.00007117102,0.000006167882,0.0001042519,0.00007963002,0.00001559291],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001945187,"about_ca_system_score_gemma":0.0001236353,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00003820029,"about_ca_topic_score_gemma":0.00002282025,"domain_scores_codex":[0.9985276,0.00006784542,0.0003720476,0.0005574068,0.0001665869,0.0003084842],"domain_scores_gemma":[0.9992104,0.000015449,0.00008704706,0.0004760734,0.00008691016,0.0001241478],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0003574872,0.00006161437,0.0007022694,0.000182605,0.0001160216,0.000006729226,0.00003437032,0.00005241094,0.9939969,0.00000680622,0.001468134,0.003014687],"study_design_scores_gemma":[0.0004518618,0.0002937964,0.002038886,0.0001734887,0.00007343155,0.000001564974,0.00001745722,0.000443715,0.9017667,7.262904e-7,0.09451368,0.0002246516],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.7242423,0.00003257204,0.001480281,0.0005606175,0.000385439,0.272636,0.0002015151,0.0001179296,0.0003433342],"genre_scores_gemma":[0.8806785,9.766943e-7,0.005996091,0.0001718981,0.0002896783,0.1125485,0.0001305846,0.00004361273,0.0001401206],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1600875,"threshold_uncertainty_score":0.77921,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01204865817108594,"score_gpt":0.3081105581292291,"score_spread":0.2960618999581432,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}