{"id":"W4392927811","doi":"10.17504/protocols.io.5qpvokxxzl4o/v1","title":"Endophytic fungal DNA extraction from seeds using Qiagen DNeasy Plant Mini v1","year":2024,"lang":"en","type":"preprint","venue":"","topic":"Plant Pathogens and Fungal Diseases","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Victoria","funders":"","keywords":"Extraction (chemistry); Plant use of endophytic fungi in defense; DNA extraction; Biology; Botany; Horticulture; Chromatography; Chemistry; Polymerase chain reaction; Gene; Biochemistry","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001808824,0.00331063,0.003649786,0.002976179,0.001744737,0.001157271,0.002383781,0.001338843,0.02304175],"category_scores_gemma":[0.001596664,0.001614159,0.001651917,0.002421194,0.0009337586,0.0006846436,0.001741092,0.004264155,0.02821098],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006316749,"about_ca_system_score_gemma":0.001445717,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002468524,"about_ca_topic_score_gemma":0.005802889,"domain_scores_codex":[0.998481,0.0001676495,0.0001470077,0.0005560829,0.0004063792,0.0002419027],"domain_scores_gemma":[0.9990729,0.0002103776,0.00008053678,0.000265446,0.0002247652,0.0001459796],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002001613,0.0001278851,0.0004300627,0.001482183,0.00004740723,0.0004377348,0.0002225776,0.0002705319,0.9677055,0.00083726,0.006972344,0.02126629],"study_design_scores_gemma":[0.0002925723,0.0008511172,0.01109077,0.0004097247,0.0003514485,0.001919333,0.0001959031,0.003262117,0.5897233,0.002245244,0.3894212,0.0002373239],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.1080156,0.01072312,0.6849841,0.0006979651,0.001597786,0.01656415,0.1142875,0.02606004,0.0370698],"genre_scores_gemma":[0.07974897,0.007914453,0.5565361,0.001042844,0.0004130978,0.01105098,0.2607068,0.007069791,0.07551694],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.02304175,"threshold_uncertainty_score":0.07708234,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02353103074399525,"score_gpt":0.2649872504239877,"score_spread":0.2414562196799924,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}