{"id":"W4393045409","doi":"10.1002/cpz1.1007","title":"Optimized Recombinant Expression and Purification of the SARS‐CoV‐2 Polymerase Complex","year":2024,"lang":"en","type":"article","venue":"Current Protocols","topic":"SARS-CoV-2 and COVID-19 Research","field":"Medicine","cited_by":1,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Windsor","funders":"","keywords":"Recombinant DNA; Sf9; Biology; Polymerase; Fusion protein; RNA polymerase; Escherichia coli; Maltose-binding protein; T7 RNA polymerase; Computational biology; Cell biology; Biochemistry; Enzyme; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003180818,0.0001275086,0.000226542,0.0001040026,0.00007519842,0.00005867297,0.0001652442,0.00005842735,0.00001917264],"category_scores_gemma":[0.0001128495,0.00007609101,0.00009910025,0.0002742237,0.0001291843,0.00008669467,0.0001197847,0.000258572,0.00001945027],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00004540686,"about_ca_system_score_gemma":0.0002076396,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001165676,"about_ca_topic_score_gemma":4.323677e-7,"domain_scores_codex":[0.9987548,0.0001116151,0.0002984218,0.0002919939,0.0003512532,0.0001919051],"domain_scores_gemma":[0.9993125,0.00007647352,0.00006928973,0.0004345896,0.00007961793,0.00002751308],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000378737,0.0002967915,0.001705154,0.001460052,0.00001667468,0.00000532393,0.0002210851,9.238419e-8,0.9384398,0.0004113154,0.004479217,0.0525858],"study_design_scores_gemma":[0.001622178,0.0001347575,0.001578187,0.001981663,0.00001964973,0.00001438942,0.00001777425,0.001268113,0.7146383,0.0002656286,0.2783791,0.00008029613],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.6388639,0.005046086,0.002280682,0.003720197,0.0009831422,0.3458903,0.00005773204,0.0003559826,0.00280208],"genre_scores_gemma":[0.6951966,0.00003591887,0.001411772,0.003275212,0.0004321437,0.2993213,0.00002608421,0.0001019543,0.0001989675],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.2738999,"threshold_uncertainty_score":0.3102901,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.1500947464055091,"score_gpt":0.4491037190867512,"score_spread":0.2990089726812421,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}