{"id":"W4393045409","doi":"10.1002/cpz1.1007","title":"Optimized Recombinant Expression and Purification of the SARS‐CoV‐2 Polymerase Complex","year":2024,"lang":"en","type":"article","venue":"Current Protocols","topic":"SARS-CoV-2 and COVID-19 Research","field":"Medicine","cited_by":1,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Windsor","funders":"","keywords":"Recombinant DNA; Sf9; Biology; Polymerase; Fusion protein; RNA polymerase; Escherichia coli; Maltose-binding protein; T7 RNA polymerase; Computational biology; Cell biology; Biochemistry; Enzyme; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001269314,0.001211213,0.001260103,0.0008558354,0.0008642771,0.0007391991,0.001120716,0.0006546155,0.006003055],"category_scores_gemma":[0.001088294,0.0008698903,0.0006678995,0.001369014,0.0003348904,0.0005366144,0.0006748529,0.002012216,0.009613633],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006538261,"about_ca_system_score_gemma":0.001292248,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001393086,"about_ca_topic_score_gemma":0.00299333,"domain_scores_codex":[0.9986264,0.0002339069,0.0002029017,0.0002296802,0.0005245287,0.0001827487],"domain_scores_gemma":[0.9995914,0.00006061578,0.00004213586,0.00008405441,0.0001801257,0.00004162325],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001542142,0.0001409344,0.0002072404,0.0006789481,0.00002473171,0.0001713552,0.0001335168,0.0004894563,0.9817329,0.001246245,0.005479453,0.009541054],"study_design_scores_gemma":[0.00008022463,0.0002686472,0.001708684,0.0001099223,0.00005307745,0.0005078526,0.00007987185,0.001755103,0.8535261,0.0005053664,0.1413192,0.00008594961],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.3542204,0.01137701,0.4593967,0.001355933,0.001387336,0.01860069,0.1040261,0.00780671,0.04182923],"genre_scores_gemma":[0.2241698,0.0130177,0.4453542,0.0006745338,0.0002064133,0.01776575,0.2297882,0.00216855,0.066855],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.006003055,"threshold_uncertainty_score":0.02008224,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.1500947464055091,"score_gpt":0.4491037190867512,"score_spread":0.2990089726812421,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}