{"id":"W4393162098","doi":"10.1002/ange.202404069","title":"A Sensitive Technique Unravels the Kinetics of Activation and <i>Trans</i> ‐Cleavage of CRISPR‐Cas Systems","year":2024,"lang":"en","type":"article","venue":"Angewandte Chemie","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Alberta","funders":"Natural Sciences and Engineering Research Council of Canada; Canadian Institutes of Health Research","keywords":"Trans-activating crRNA; Kinetics; CRISPR; Chemistry; Ribonucleoprotein; Cleavage (geology); Activator (genetics); Ternary complex; RNA; Dissociation constant; Reaction rate constant; Biophysics; Stereochemistry; Enzyme; Biochemistry; Genome editing; Biology; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001639811,0.001110883,0.0008885069,0.0008873018,0.000477881,0.001213402,0.001087854,0.001560333,0.001206605],"category_scores_gemma":[0.002557357,0.001029174,0.0006568678,0.0005729737,0.0009495644,0.001391076,0.0007507388,0.003518281,0.001029741],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.001374108,"about_ca_system_score_gemma":0.001041081,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001046666,"about_ca_topic_score_gemma":0.0009408601,"domain_scores_codex":[0.998031,0.0002909637,0.0001279118,0.0006800973,0.0007175631,0.0001525299],"domain_scores_gemma":[0.998244,0.0006851084,0.000405456,0.0002699426,0.0002856553,0.0001098393],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003192092,0.00002763126,0.0002896252,0.0001140318,0.00001435331,0.00002917291,0.00003965136,0.0003556437,0.9922504,0.001133105,0.0002271948,0.005487377],"study_design_scores_gemma":[0.000004736265,0.00006028656,0.0007447916,0.00001365174,0.00001432233,0.0001210718,0.00001984717,0.005336832,0.990142,0.0004490041,0.003063668,0.00002984325],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"empirical","genre_scores_codex":[0.1863,0.005188119,0.7985997,0.0005662591,0.000501851,0.0002148144,0.001050455,0.002338886,0.005240054],"genre_scores_gemma":[0.6646708,0.004176794,0.3195701,0.0006757713,0.0001054273,0.0006634627,0.0008055439,0.0004019314,0.008930008],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.001639811,"threshold_uncertainty_score":0.00996989,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008347912256804828,"score_gpt":0.2714212092813662,"score_spread":0.2630732970245614,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}