{"id":"W4393281926","doi":"10.1016/j.scr.2024.103408","title":"Generation of three isogenic gene-edited Huntington’s disease human embryonic stem cell lines with DOX-inducible NGN2 expression cassette in the AAVS1 safe locus","year":2024,"lang":"en","type":"article","venue":"Stem Cell Research","topic":"Genetic Neurodegenerative Diseases","field":"Neuroscience","cited_by":3,"is_retracted":false,"has_abstract":true,"ca_institutions":"BC Children's Hospital; University of British Columbia","funders":"University of British Columbia; H. Lundbeck A/S; Lundbeckfonden; Aase og Ejnar Danielsens Fond; Hørslev-Fonden; BC Children's Hospital; Michael Smith Health Research BC; Novo Nordisk Fonden; Københavns Universitet; Novo Nordisk; Huntington Society of Canada","keywords":"Biology; Huntingtin; Embryonic stem cell; Huntington's disease; Molecular biology; Doxycycline; Stem cell; Homeobox protein NANOG; Cell biology; Exon; Gene; Genetics; Induced pluripotent stem cell; Pathology; Disease","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004113947,0.0004479798,0.0004197687,0.0004902665,0.0002094371,0.000443679,0.00058175,0.0004044532,0.001903745],"category_scores_gemma":[0.0001954357,0.0002684137,0.0002784594,0.0002592831,0.0002860141,0.0002201875,0.0003612181,0.0008421234,0.0007269477],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002508283,"about_ca_system_score_gemma":0.0002522086,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004670499,"about_ca_topic_score_gemma":0.00117795,"domain_scores_codex":[0.9996734,0.00002515198,0.00006437629,0.00006455323,0.0001405106,0.00003207112],"domain_scores_gemma":[0.9998385,0.0000188203,0.00003285822,0.00003125848,0.00003367531,0.00004482808],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001207079,0.0001101696,0.0004114565,0.00005817775,0.00001291192,0.0001670081,0.0001358327,0.0001114614,0.9943274,0.0006652879,0.0002127258,0.003666951],"study_design_scores_gemma":[0.00004954206,0.0002907494,0.00275436,0.00001081822,0.00005162852,0.0006145102,0.00005975697,0.001228784,0.982863,0.0001253803,0.01193576,0.00001564113],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9350172,0.0007290171,0.05242852,0.0001360229,0.0002337096,0.0009616879,0.003840583,0.0004498087,0.006203457],"genre_scores_gemma":[0.9148733,0.001380106,0.0505757,0.0001890287,0.00002995935,0.001392112,0.009960285,0.0002293055,0.02137019],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001903745,"threshold_uncertainty_score":0.006368637,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.1567127692088657,"score_gpt":0.3585691873294604,"score_spread":0.2018564181205947,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}