{"id":"W4393353400","doi":"10.1111/febs.17125","title":"Ni( <scp>II</scp> )‐binding affinity of <i>Cc</i> NikZ‐II and its homologs: the role of the <scp>HH</scp> ‐prong and variable loop revealed by structural and mutational studies","year":2024,"lang":"en","type":"article","venue":"FEBS Journal","topic":"Trace Elements in Health","field":"Nursing","cited_by":1,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Calgary; University of Toronto","funders":"Ontario Ministry of Research and Innovation; Ontario Ministry of Economic Development, Job Creation and Trade; Ministero dello Sviluppo Economico; Lawrence Livermore National Laboratory; U.S. Department of Energy","keywords":"Chemistry; Mutagenesis; Binding site; Affinities; Stereochemistry; Plasma protein binding; Binding affinities; Biophysics; Biochemistry; Crystallography; Mutation; Biology; Receptor; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00006009794,0.0002249987,0.0002104436,0.0001072552,0.00012725,0.0002303512,0.0002935183,0.0001515106,0.0009465249],"category_scores_gemma":[0.0001486466,0.00009943033,0.0001407528,0.0001169431,0.0002007062,0.0001145306,0.0001808711,0.0002717133,0.0002322801],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000439992,"about_ca_system_score_gemma":0.0001731884,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.004910282,"about_ca_topic_score_gemma":0.003928514,"domain_scores_codex":[0.9999409,0.000004654013,0.000004059926,0.0000158473,0.00002259753,0.00001189391],"domain_scores_gemma":[0.9999535,0.000008924198,0.00001017745,0.000003053846,0.000008456452,0.00001589467],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002096588,0.0000214248,0.003757183,0.0000713703,0.00001373543,0.0001183226,0.00005810095,0.0003504005,0.9933804,0.0001842223,0.00007663783,0.001758624],"study_design_scores_gemma":[0.00001525442,0.00008687218,0.02575243,0.000006470261,0.00001400649,0.000461473,0.000118167,0.004174271,0.9673306,0.00009377307,0.001935803,0.00001087043],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9984707,0.0002387956,0.0006526795,0.00002594209,0.00000217306,0.000002549698,0.0001105518,0.00001853982,0.0004781346],"genre_scores_gemma":[0.9980891,0.0001036941,0.000870217,0.00001173635,0.00000105966,0.000002973246,0.0004295907,0.000009510176,0.0004821293],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.004910282,"threshold_uncertainty_score":0.00976342,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01675828697597643,"score_gpt":0.2903173825740653,"score_spread":0.2735590955980888,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}