{"id":"W4393353400","doi":"10.1111/febs.17125","title":"Ni( <scp>II</scp> )‐binding affinity of <i>Cc</i> NikZ‐II and its homologs: the role of the <scp>HH</scp> ‐prong and variable loop revealed by structural and mutational studies","year":2024,"lang":"en","type":"article","venue":"FEBS Journal","topic":"Trace Elements in Health","field":"Nursing","cited_by":1,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Calgary; University of Toronto","funders":"Ontario Ministry of Research and Innovation; Ontario Ministry of Economic Development, Job Creation and Trade; Ministero dello Sviluppo Economico; Lawrence Livermore National Laboratory; U.S. Department of Energy","keywords":"Chemistry; Mutagenesis; Binding site; Affinities; Stereochemistry; Plasma protein binding; Binding affinities; Biophysics; Biochemistry; Crystallography; Mutation; Biology; Receptor; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["sts"],"consensus_categories":[],"category_scores_codex":[0.001438478,0.0002487405,0.0003996996,0.0001179717,0.001335473,0.0001312115,0.0002499543,0.0001178448,0.000005662592],"category_scores_gemma":[0.001157409,0.0001574943,0.00005991002,0.0003151625,0.0003446731,0.0003924333,0.0003075249,0.0008235878,6.874038e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001078589,"about_ca_system_score_gemma":0.0001239972,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00002561002,"about_ca_topic_score_gemma":0.0000211152,"domain_scores_codex":[0.997726,0.0003663529,0.0006453374,0.0003052531,0.0005044427,0.000452673],"domain_scores_gemma":[0.9973039,0.001726638,0.0004708772,0.0001728871,0.0002044796,0.0001212335],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"observational","study_design_gemma":"observational","study_design_scores_codex":[0.0002221012,0.00120116,0.4805428,0.005293963,0.004234781,0.00009611773,0.1613802,0.0005534757,0.1617243,0.01699165,0.1280856,0.03967388],"study_design_scores_gemma":[0.01939845,0.005062117,0.4267751,0.007884153,0.003091548,0.011626,0.1496161,0.008012803,0.1841985,0.1505528,0.03306502,0.0007174503],"study_design_candidate":"observational","study_design_consensus":"observational","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9494166,0.04792156,0.000009912667,0.0006718162,0.001161326,0.0003640138,0.0001651058,0.00002391635,0.0002657329],"genre_scores_gemma":[0.9976889,0.0006108665,0.0006765075,0.0001254682,0.0002567106,0.000009187397,0.000004583611,0.00003173596,0.0005959834],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1335611,"threshold_uncertainty_score":0.9999647,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01675828697597643,"score_gpt":0.2903173825740653,"score_spread":0.2735590955980888,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}