{"id":"W4393389014","doi":"10.1111/mmi.15256","title":"<i>Streptococcus pyogenes</i> Cas9 ribonucleoprotein delivery for efficient, rapid and marker‐free gene editing in <i>Trypanosoma</i> and <i>Leishmania</i>","year":2024,"lang":"en","type":"article","venue":"Molecular Microbiology","topic":"Trypanosoma species research and implications","field":"Medicine","cited_by":15,"is_retracted":false,"has_abstract":true,"ca_institutions":"Centre hospitalier de l'Université Laval","funders":"Université de Bordeaux; Canadian Institutes of Health Research; Centre National de la Recherche Scientifique; Fondation pour la Recherche Médicale; Agence Nationale de la Recherche","keywords":"Biology; Cas9; Trypanosoma cruzi; Trypanosoma brucei; CRISPR; Genome editing; Leishmania; Virology; Plasmid; Kinetoplastida; Genome; Guide RNA; Gene; Genetics; Parasite hosting","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001494027,0.0002674703,0.0002267941,0.0001148832,0.0001314795,0.0003133227,0.0002945954,0.0003831307,0.001058594],"category_scores_gemma":[0.0001622682,0.0001521318,0.0001928631,0.0001154066,0.0002233097,0.0002097047,0.0002791388,0.0005459847,0.0004867839],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000268543,"about_ca_system_score_gemma":0.0001977065,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007409567,"about_ca_topic_score_gemma":0.0007737653,"domain_scores_codex":[0.9998417,0.00002593168,0.00001616908,0.00003812171,0.00005663313,0.00002140621],"domain_scores_gemma":[0.999933,0.00001101057,0.00002603413,0.000009580647,0.000007234556,0.00001314517],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003656135,0.000008199435,0.00004143612,0.00004990901,0.000004042735,0.00005374053,0.00001548158,0.0001086748,0.997196,0.0003605511,0.0001426371,0.001982753],"study_design_scores_gemma":[0.000005851608,0.00005608815,0.0003268064,0.000004611567,0.000007682907,0.000243706,0.000008255852,0.001830026,0.9907067,0.00004464289,0.006759293,0.000006292262],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8854614,0.003594546,0.09639263,0.0004495435,0.0002098043,0.000200032,0.001015399,0.001456474,0.01122007],"genre_scores_gemma":[0.9661089,0.001247268,0.02442641,0.00007930352,0.00001889197,0.00007103876,0.0007787761,0.0001403609,0.007129028],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001058594,"threshold_uncertainty_score":0.00354141,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.009231545040324935,"score_gpt":0.2504162131641317,"score_spread":0.2411846681238068,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}