{"id":"W4393940733","doi":"10.1002/cpz1.1009","title":"Advancing Genetic Alphabet Expansion: Synthesis of 7‐(2‐Thienyl)‐Imidazo[4,5‐b]pyridine (Ds) and 4‐(4‐Pentyne‐1,2‐diol)‐1‐Propynyl‐2‐Nitropyrrole (Diol‐Px) for Use in Replicable Unnatural Base Pairs for PCR Applications","year":2024,"lang":"en","type":"article","venue":"Current Protocols","topic":"Advanced biosensing and bioanalysis techniques","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"Xenon Pharmaceuticals (Canada)","funders":"","keywords":"Deoxyribonucleoside; Deoxyribonucleosides; Chemistry; DNA; Combinatorial chemistry; Phosphoramidite; Oligonucleotide; Stereochemistry; Biochemistry","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001718686,0.0003996811,0.000188235,0.0002522962,0.0001757861,0.0001933785,0.0003355497,0.0003056571,0.003963247],"category_scores_gemma":[0.0002583513,0.0002382419,0.0001957921,0.0003142605,0.0001654055,0.0003606134,0.0003579076,0.0007690192,0.002159436],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003537199,"about_ca_system_score_gemma":0.0005304613,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004292302,"about_ca_topic_score_gemma":0.0009456952,"domain_scores_codex":[0.9998757,0.00001410058,0.00001218166,0.00003195243,0.00003946617,0.00002665647],"domain_scores_gemma":[0.9999107,0.00001537407,0.00001949566,0.00001783794,0.00001719205,0.00001934891],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005100707,0.00003434336,0.00007818024,0.0003141267,0.000005981663,0.0002186336,0.00007303696,0.0007473131,0.9740413,0.002340893,0.0006875063,0.02140777],"study_design_scores_gemma":[0.00001115198,0.0001226569,0.0002018755,0.00001234828,0.000005780265,0.0002684448,0.00001428657,0.0009970231,0.9798048,0.0002502059,0.01830026,0.00001136621],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.6597607,0.008193433,0.2509,0.0005831767,0.0005387082,0.0008851863,0.002621117,0.002760429,0.0737572],"genre_scores_gemma":[0.8357196,0.006507653,0.1323734,0.0002720152,0.00004678654,0.0005868445,0.001668746,0.0002480953,0.02257687],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.003963247,"threshold_uncertainty_score":0.01325846,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02705874564142028,"score_gpt":0.344204158210123,"score_spread":0.3171454125687027,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}