{"id":"W4393942316","doi":"10.1016/j.bej.2024.109310","title":"Immobilization of alcalase on polydopamine modified magnetic particles","year":2024,"lang":"en","type":"article","venue":"Biochemical Engineering Journal","topic":"Enzyme Catalysis and Immobilization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":8,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Ottawa","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Immobilized enzyme; Chemistry; Glutaraldehyde; Enzyme; Magnetic nanoparticles; Protease; Michaelis–Menten kinetics; Chemical engineering; Chromatography; Enzyme assay; Nanotechnology; Biochemistry; Materials science; Nanoparticle","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001222113,0.0001076062,0.00009886749,0.00007571246,0.00001822738,0.00003301121,0.00008958537,0.00008756314,0.00002079825],"category_scores_gemma":[0.0001607973,0.00009313666,0.00009788356,0.0001478872,0.00002554511,0.000004979628,0.00002494097,0.00009866363,0.00000459005],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001560308,"about_ca_system_score_gemma":0.00002978519,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":4.500696e-7,"about_ca_topic_score_gemma":6.598837e-8,"domain_scores_codex":[0.9993205,0.0000113217,0.0002381725,0.0001648463,0.0001256064,0.000139526],"domain_scores_gemma":[0.9996773,0.00001487909,0.00003233997,0.0001412559,0.00005304014,0.00008119456],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002391983,0.00004342692,0.00001881988,0.00004154488,0.00002484889,0.00000302877,0.00001052597,0.007537165,0.9892533,0.0002266301,0.001078021,0.001738745],"study_design_scores_gemma":[0.0001832307,0.0002441723,0.0001676496,0.0000862475,0.00002278912,0.00004652535,0.000006314849,0.02406952,0.9722227,0.0000146799,0.002828594,0.0001075236],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9714197,0.006473457,0.0217053,0.0000612488,0.0002022431,0.00005275945,0.000007699809,0.00001991422,0.00005769882],"genre_scores_gemma":[0.9986697,0.0003262532,0.0004960199,0.00002213577,0.00029263,0.000004687579,0.00005657056,0.00002195636,0.0001100109],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.02725005,"threshold_uncertainty_score":0.3798003,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006832812773646372,"score_gpt":0.219671319811877,"score_spread":0.2128385070382306,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}