{"id":"W4393993285","doi":"10.1021/jacs.4c01419","title":"Two Iron(II), α-Ketoglutarate-Dependent Enzymes Encoded by the <i>PPZ</i> Gene Cluster of <i>Metarhizium majus</i> Enable Production of 8-Hydroxyperamine","year":2024,"lang":"en","type":"article","venue":"Journal of the American Chemical Society","topic":"Enzyme Catalysis and Immobilization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":3,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Chemistry; Gene cluster; Nonribosomal peptide; Gene; Enzyme; Biosynthesis; Stereochemistry; Biochemistry; Fungus; Botany; Biology","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004975288,0.0001493568,0.0003071281,0.0000133273,0.00006486505,0.0000178671,0.0003886245,0.00006343836,0.000009321023],"category_scores_gemma":[0.00009686996,0.00007679318,0.0005304171,0.0002943468,0.0003996664,0.00001436221,0.0001879958,0.0002231504,5.728548e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00004394193,"about_ca_system_score_gemma":0.00009416181,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00003453682,"about_ca_topic_score_gemma":0.000001145939,"domain_scores_codex":[0.9986994,0.00008411354,0.0005086293,0.0002130151,0.0003282413,0.0001665625],"domain_scores_gemma":[0.9988195,0.00002901768,0.0005630151,0.0003506334,0.000193749,0.00004409924],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00006046787,0.0001105921,0.0001211196,0.00003775123,0.0002123,1.67432e-7,0.0001763706,0.0006751698,0.9558074,0.000003187318,0.042407,0.0003884842],"study_design_scores_gemma":[0.0002470139,0.0001661219,0.0000145232,0.00002909056,0.0001774978,0.0000536433,0.0002503965,0.0002643021,0.9901057,0.00002407723,0.008573925,0.0000937464],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9934172,0.003849828,0.0004637187,0.001807051,0.0002725794,0.0001160341,0.00002320336,0.000003983251,0.00004640607],"genre_scores_gemma":[0.9964308,0.001027752,0.000543387,0.0005166552,0.0004196985,0.000004386905,0.00002672086,0.00002183491,0.001008735],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.03429827,"threshold_uncertainty_score":0.3131535,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.004962051202496459,"score_gpt":0.2318154754568265,"score_spread":0.2268534242543301,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}