{"id":"W4393993285","doi":"10.1021/jacs.4c01419","title":"Two Iron(II), α-Ketoglutarate-Dependent Enzymes Encoded by the <i>PPZ</i> Gene Cluster of <i>Metarhizium majus</i> Enable Production of 8-Hydroxyperamine","year":2024,"lang":"en","type":"article","venue":"Journal of the American Chemical Society","topic":"Enzyme Catalysis and Immobilization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":3,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Chemistry; Gene cluster; Nonribosomal peptide; Gene; Enzyme; Biosynthesis; Stereochemistry; Biochemistry; Fungus; Botany; Biology","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00008666139,0.0004963029,0.0002207204,0.0001659562,0.0001042293,0.0002493951,0.0002573159,0.0002445803,0.0004036624],"category_scores_gemma":[0.0001436563,0.0001773536,0.000245009,0.0001449109,0.0001490507,0.0001929175,0.0003539339,0.0004996181,0.0002397449],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002950173,"about_ca_system_score_gemma":0.0002618121,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004357597,"about_ca_topic_score_gemma":0.0006030535,"domain_scores_codex":[0.999881,0.0000127624,0.000008456032,0.00003563003,0.00003615359,0.00002599864],"domain_scores_gemma":[0.9999083,0.00001101298,0.00002746909,0.00001149143,0.00001192995,0.00002988001],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003686767,0.000008058511,0.0002133856,0.00001412363,0.000001925974,0.00002400455,0.000007738473,0.00002809914,0.9984136,0.00004437155,0.000009206885,0.001198662],"study_design_scores_gemma":[0.000007244649,0.00007428615,0.004217523,0.000002020351,0.000007603703,0.000220808,0.00001921946,0.0004738193,0.9939318,0.00004027314,0.001001991,0.00000344675],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9867749,0.0004855783,0.01106316,0.00007012351,0.00002161204,0.00004935509,0.0003263462,0.00009277054,0.001116096],"genre_scores_gemma":[0.9849209,0.0002724271,0.01141303,0.00002624381,0.00000486885,0.00003393132,0.0009752738,0.00002297603,0.002330341],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0004963029,"threshold_uncertainty_score":0.002140462,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.004962051202496459,"score_gpt":0.2318154754568265,"score_spread":0.2268534242543301,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}