{"id":"W4394680557","doi":"10.1016/j.omtm.2024.101249","title":"Metabolic priming of GD2 TRAC-CAR T cells during manufacturing promotes memory phenotypes while enhancing persistence","year":2024,"lang":"en","type":"article","venue":"Molecular Therapy — Methods & Clinical Development","topic":"CAR-T cell therapy research","field":"Medicine","cited_by":30,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"National Cancer Institute; Grainger Institute for Engineering, University of Wisconsin-Madison; College of Engineering, University of Wisconsin-Madison; National Institute of General Medical Sciences; Engineering Research Centers; Government of the United Kingdom; U.S. Department of Health and Human Services; National Institutes of Health; University of Wisconsin Carbone Cancer Center; St. Baldrick's Foundation; Canadian Thoracic Society; Hyundai Hope On Wheels; National Science Foundation; Michigan Council for Arts and Cultural Affairs; University of Wisconsin-Madison; Division of Graduate Education; National Heart, Lung, and Blood Institute; Wisconsin Alumni Research Foundation","keywords":"CRISPR; Chimeric antigen receptor; Priming (agriculture); Persistence (discontinuity); Cas9; Phenotype; Ribonucleoprotein; Biology; Cell biology; Computational biology; T cell; Genetics; Gene; Engineering; Immune system; RNA","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000148233,0.0003827041,0.0001908607,0.0001639805,0.0001353213,0.0004485441,0.0001885068,0.0002522932,0.001119394],"category_scores_gemma":[0.0001430139,0.0001229659,0.000249157,0.0001575095,0.0001556953,0.0002692208,0.0002786145,0.0003657727,0.0004140834],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002390811,"about_ca_system_score_gemma":0.0002306943,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000316262,"about_ca_topic_score_gemma":0.0006448326,"domain_scores_codex":[0.9998628,0.00002504653,0.00001159951,0.00003514597,0.00003572662,0.00002972483],"domain_scores_gemma":[0.9998903,0.00001908637,0.00004207084,0.00001522586,0.0000178579,0.00001535237],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004467531,0.00001227522,0.0001359434,0.00002191642,0.000002258655,0.00002454602,0.00001085862,0.0001515398,0.9975873,0.00007131324,0.00002728729,0.001910026],"study_design_scores_gemma":[0.000003696711,0.0001191321,0.0003783141,0.000001985847,0.000006054572,0.00006431688,0.000007163214,0.0003845682,0.9980519,0.00001767165,0.0009624733,0.000002716354],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9752797,0.000869233,0.0208415,0.00009622639,0.00002467704,0.00006891893,0.0002446543,0.0003106098,0.002264473],"genre_scores_gemma":[0.9817758,0.0005143727,0.01552802,0.00005346665,0.000008869548,0.00007741978,0.0002848295,0.00006575664,0.001691569],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001119394,"threshold_uncertainty_score":0.003744781,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.102722372450158,"score_gpt":0.4156942167703084,"score_spread":0.3129718443201504,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}