{"id":"W4394680557","doi":"10.1016/j.omtm.2024.101249","title":"Metabolic priming of GD2 TRAC-CAR T cells during manufacturing promotes memory phenotypes while enhancing persistence","year":2024,"lang":"en","type":"article","venue":"Molecular Therapy — Methods & Clinical Development","topic":"CAR-T cell therapy research","field":"Medicine","cited_by":30,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"National Cancer Institute; Grainger Institute for Engineering, University of Wisconsin-Madison; College of Engineering, University of Wisconsin-Madison; National Institute of General Medical Sciences; Engineering Research Centers; Government of the United Kingdom; U.S. Department of Health and Human Services; National Institutes of Health; University of Wisconsin Carbone Cancer Center; St. Baldrick's Foundation; Canadian Thoracic Society; Hyundai Hope On Wheels; National Science Foundation; Michigan Council for Arts and Cultural Affairs; University of Wisconsin-Madison; Division of Graduate Education; National Heart, Lung, and Blood Institute; Wisconsin Alumni Research Foundation","keywords":"CRISPR; Chimeric antigen receptor; Priming (agriculture); Persistence (discontinuity); Cas9; Phenotype; Ribonucleoprotein; Biology; Cell biology; Computational biology; T cell; Genetics; Gene; Engineering; Immune system; RNA","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow","insufficient_payload"],"consensus_categories":[],"category_scores_codex":[0.006162406,0.0004833744,0.001201852,0.0003807355,0.0001564865,0.00009031421,0.0004187537,0.0002923221,0.001169623],"category_scores_gemma":[0.0003121774,0.0004087069,0.0006984647,0.0004513127,0.0002213739,0.00011768,0.0002072754,0.0009724243,0.00005632213],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001748165,"about_ca_system_score_gemma":0.0007741668,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001792656,"about_ca_topic_score_gemma":0.000002841908,"domain_scores_codex":[0.9944297,0.001162798,0.001622641,0.001081296,0.0009413783,0.0007621679],"domain_scores_gemma":[0.9974146,0.00106271,0.000205383,0.0007660294,0.0001810876,0.0003701825],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002584871,0.0001782683,0.00005680027,0.0003703331,0.0007425174,0.000131545,0.001922023,0.00001336443,0.6013927,0.00001075763,0.000003557737,0.3949197],"study_design_scores_gemma":[0.001272114,0.000236056,0.004516756,0.0007074633,0.0001421975,0.00003833122,0.0002908505,0.0001490895,0.9771777,0.0001189312,0.01494655,0.0004040019],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.9510678,0.01386289,0.03167022,0.0002759854,0.0006469743,0.001203999,0.000002535784,0.0001972026,0.001072397],"genre_scores_gemma":[0.4732684,0.002774651,0.5196941,0.0003433469,0.000268854,0.000127607,0.00001277179,0.000180094,0.003330173],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.4880239,"threshold_uncertainty_score":0.9998365,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.102722372450158,"score_gpt":0.4156942167703084,"score_spread":0.3129718443201504,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}