{"id":"W4394912734","doi":"10.1038/s41596-024-00979-z","title":"Luciferase- and HaloTag-based reporter assays to measure small-molecule-induced degradation pathway in living cells","year":2024,"lang":"en","type":"review","venue":"Nature Protocols","topic":"Protein Degradation and Inhibitors","field":"Biochemistry, Genetics and Molecular Biology","cited_by":32,"is_retracted":false,"has_abstract":false,"ca_institutions":"Structural Genomics Consortium","funders":"Deutsche Forschungsgemeinschaft","keywords":"Small molecule; Computational biology; CRISPR; HEK 293 cells; High-throughput screening; Synthetic biology; Proteomics; Cell biology; Protein degradation; Ternary complex; Cell culture; Biology; Chemistry; Molecular biology; Biochemistry; Genetics; Gene; Enzyme","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001762985,0.002442484,0.001810238,0.002589016,0.0003830881,0.001347689,0.002496804,0.001474063,0.003435496],"category_scores_gemma":[0.000927415,0.001024365,0.0009345943,0.002434826,0.001154203,0.002004845,0.0009896298,0.004639568,0.006381668],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.002148937,"about_ca_system_score_gemma":0.0015206,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001536426,"about_ca_topic_score_gemma":0.002733418,"domain_scores_codex":[0.9993594,0.00007925662,0.00005042106,0.0000990057,0.0003436954,0.00006820227],"domain_scores_gemma":[0.9994279,0.0002248974,0.00006701769,0.00004026754,0.0001951421,0.00004467271],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"design_other","study_design_gemma":"not_applicable","study_design_scores_codex":[0.0001690539,0.0002056494,0.0001664071,0.01351584,0.000106352,0.0001917057,0.00006797376,0.001202667,0.1176537,0.02026091,0.06403622,0.7824234],"study_design_scores_gemma":[0.0000309141,0.000114162,0.0004445416,0.0008689126,0.00007505929,0.0005017821,0.00002186063,0.0005912336,0.06360848,0.00321126,0.9304686,0.00006310568],"study_design_candidate":"not_applicable","study_design_consensus":null,"genre_codex":"review","genre_gemma":"protocol","genre_scores_codex":[0.0008018772,0.9571298,0.02982241,0.001138276,0.001995296,0.0001605173,0.00057869,0.0005186693,0.007854478],"genre_scores_gemma":[0.00302371,0.967086,0.01500687,0.0008205731,0.0003334365,0.0003193336,0.001007792,0.0001025649,0.01229986],"genre_candidate":"protocol","genre_consensus":null,"teacher_disagreement_score":0.003435496,"threshold_uncertainty_score":0.01559174,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.04151310621609912,"score_gpt":0.3417986249798247,"score_spread":0.3002855187637256,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}