{"id":"W4395465087","doi":"10.1007/s11105-024-01459-0","title":"Development of a Csy4-Processing TRV-Based CRISPR/Cas9 Genome Editing System in Nicotiana benthamiana","year":2024,"lang":"en","type":"article","venue":"Plant Molecular Biology Reporter","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":3,"is_retracted":false,"has_abstract":true,"ca_institutions":"Agriculture and Agri-Food Canada","funders":"Agriculture and Agri-Food Canada; National Natural Science Foundation of China","keywords":"Nicotiana benthamiana; CRISPR; Biology; Genome editing; Cas9; Computational biology; Genome; Guide RNA; Genetics; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003781479,0.0002332182,0.0002811225,0.0001917032,0.0000388405,0.0000229183,0.0001539565,0.0002343315,0.000007783003],"category_scores_gemma":[0.0000335363,0.0002213842,0.0001098893,0.0001546379,0.00004929557,0.000003091586,0.0000769203,0.0001262388,0.000003600361],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00004183808,"about_ca_system_score_gemma":0.0002137751,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001200509,"about_ca_topic_score_gemma":0.00004639122,"domain_scores_codex":[0.9982839,0.00004078645,0.0006471393,0.0005508022,0.0001153313,0.0003620789],"domain_scores_gemma":[0.9994688,0.00001032721,0.0001168352,0.0002875484,0.00004385635,0.00007263306],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001487572,0.0000261712,0.002023597,0.0003789114,0.00008376377,0.0003267375,0.0001281671,0.0004548088,0.9939949,0.00005246746,0.00003616088,0.002479465],"study_design_scores_gemma":[0.0004465122,0.0001172473,0.00253841,0.0004435034,0.00005876859,0.0004830471,0.0002750129,0.006263099,0.9668657,0.0000112627,0.02200304,0.000494396],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.7885171,0.005342613,0.2048884,0.00003658954,0.0003438992,0.0002031143,0.00003282273,0.00004745679,0.0005879328],"genre_scores_gemma":[0.9921229,0.00000983928,0.007138685,0.00005566878,0.0001240259,0.00003721017,0.000451189,0.00003577247,0.00002469319],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.2036058,"threshold_uncertainty_score":0.9027787,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008568074725878604,"score_gpt":0.284130632212001,"score_spread":0.2755625574861224,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}