{"id":"W4396705467","doi":"10.1002/ange.202407862","title":"Innentitelbild: A Sensitive Technique Unravels the Kinetics of Activation and <i>Trans</i> ‐Cleavage of CRISPR‐Cas Systems (Angew. Chem. 22/2024)","year":2024,"lang":"de","type":"article","venue":"Angewandte Chemie","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":2,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Alberta","funders":"","keywords":"Kinetics; Chemistry; Nucleic acid; CRISPR; Ribonucleoprotein; Cleavage (geology); Reaction rate constant; Dissociation constant; RNA; Biophysics; Dissociation (chemistry); Stereochemistry; Receptor–ligand kinetics; Biochemistry; Biology; Physical chemistry; Physics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0009998545,0.0009002725,0.000621378,0.001182485,0.000631584,0.001115454,0.001277391,0.00135963,0.00522179],"category_scores_gemma":[0.0007356839,0.0006585455,0.0004411385,0.0005357282,0.0010422,0.001498191,0.001295162,0.002997307,0.003005042],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0009058879,"about_ca_system_score_gemma":0.000656108,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0009379744,"about_ca_topic_score_gemma":0.001749252,"domain_scores_codex":[0.9989269,0.0001447951,0.00003992958,0.0003392963,0.0004439757,0.0001050753],"domain_scores_gemma":[0.9995002,0.0002082211,0.0001014496,0.00008457984,0.00005309539,0.0000524449],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001042578,0.00003629424,0.0003505902,0.0001354708,0.00001395345,0.0001949961,0.000124626,0.0003474766,0.9555735,0.00703312,0.005472066,0.03061376],"study_design_scores_gemma":[0.00001778786,0.00007772414,0.0005651955,0.00002527085,0.00001376573,0.0005158265,0.00005338097,0.006548956,0.9526849,0.002233031,0.03720677,0.00005728832],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"empirical","genre_scores_codex":[0.1468564,0.01255072,0.7771324,0.004004334,0.002448674,0.0002504567,0.003281791,0.01119028,0.04228492],"genre_scores_gemma":[0.4155715,0.009351656,0.4907656,0.002489374,0.0004074553,0.0007237733,0.002974436,0.001867448,0.07584874],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.00522179,"threshold_uncertainty_score":0.01746863,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007949954743111304,"score_gpt":0.2689491574846914,"score_spread":0.2609992027415802,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}