{"id":"W4396856448","doi":"10.3390/fermentation10050250","title":"High-Level Bio-Based Production of Coproporphyrin in Escherichia coli","year":2024,"lang":"en","type":"article","venue":"Fermentation","topic":"Porphyrin Metabolism and Disorders","field":"Biochemistry, Genetics and Molecular Biology","cited_by":5,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Waterloo","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Escherichia coli; Metabolic engineering; Heterologous; Plasmid; Operon; Enzyme; Bioreactor; Synthetic biology; Biochemistry; Biology; Strain (injury); Heterologous expression; Chemistry; Gene; Computational biology; Recombinant DNA","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002591531,0.0004782234,0.0003739906,0.0002546063,0.0001314299,0.0005122625,0.0003093687,0.0003417297,0.0003245772],"category_scores_gemma":[0.0001904987,0.0001333782,0.0002316038,0.0003297007,0.000164555,0.0002735072,0.0004445195,0.0006419735,0.0004283928],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003316367,"about_ca_system_score_gemma":0.0002803037,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000648737,"about_ca_topic_score_gemma":0.0007551059,"domain_scores_codex":[0.9997026,0.00007289134,0.00003422101,0.00005045252,0.0000987221,0.0000411338],"domain_scores_gemma":[0.9998988,0.0000224091,0.00002356168,0.00002127839,0.00001678293,0.00001706335],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001931442,0.00003133531,0.0001131442,0.00003475605,0.000004552393,0.00005330783,0.000009715561,0.0002244615,0.997451,0.0001044491,0.00002423436,0.001929772],"study_design_scores_gemma":[0.000004659732,0.00009335155,0.0006041452,0.000005144559,0.000009725451,0.0001247202,0.00002053113,0.001281373,0.9961979,0.00003973359,0.001612988,0.000005720499],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9276118,0.001369248,0.06597906,0.000389418,0.00007124397,0.0002006188,0.001140267,0.0004535349,0.0027847],"genre_scores_gemma":[0.9451404,0.001550002,0.04848087,0.0000830285,0.00001126768,0.00008636349,0.001420736,0.0001034364,0.003123775],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.000648737,"threshold_uncertainty_score":0.00240624,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01276086898211279,"score_gpt":0.2622397984970778,"score_spread":0.249478929514965,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}