{"id":"W4396856448","doi":"10.3390/fermentation10050250","title":"High-Level Bio-Based Production of Coproporphyrin in Escherichia coli","year":2024,"lang":"en","type":"article","venue":"Fermentation","topic":"Porphyrin Metabolism and Disorders","field":"Biochemistry, Genetics and Molecular Biology","cited_by":5,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Waterloo","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Escherichia coli; Metabolic engineering; Heterologous; Plasmid; Operon; Enzyme; Bioreactor; Synthetic biology; Biochemistry; Biology; Strain (injury); Heterologous expression; Chemistry; Gene; Computational biology; Recombinant DNA","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001207588,0.00007341351,0.00007289136,0.00007221481,0.00001505143,0.0000110505,0.00004712004,0.00005359983,0.00002746079],"category_scores_gemma":[0.00002559787,0.00007222417,0.00003148112,0.0001777662,0.00003542261,0.000006135946,0.00001228509,0.00003571755,0.000009921744],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001122583,"about_ca_system_score_gemma":0.00007632611,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00008419871,"about_ca_topic_score_gemma":0.00003919869,"domain_scores_codex":[0.9994263,0.00003330478,0.0001550763,0.0002048908,0.00008899139,0.00009145217],"domain_scores_gemma":[0.9998016,0.000002265552,0.00003632634,0.0001141883,0.00002752211,0.00001814661],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000707632,0.00006897382,0.002905562,0.00006904762,0.00001038954,9.367172e-7,0.00005288933,0.0004382308,0.9920155,0.0002884437,0.001180094,0.002899152],"study_design_scores_gemma":[0.0004356859,0.00008963436,0.1032058,0.0000241636,0.0000134404,5.362934e-7,0.0001039431,0.0001673772,0.8939631,0.0002826965,0.001616079,0.00009754591],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9968791,0.0004239872,0.001652489,0.0001792283,0.0005195882,0.0001907347,0.00001700968,0.00001209514,0.0001257798],"genre_scores_gemma":[0.9971454,0.00006722774,0.001878353,0.00006947736,0.00008683684,0.00003448302,0.0003723478,0.00001096605,0.0003349184],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1003002,"threshold_uncertainty_score":0.2945217,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01276086898211279,"score_gpt":0.2622397984970778,"score_spread":0.249478929514965,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}