{"id":"W4396927795","doi":"10.1002/pro.5021","title":"Protein purification via consecutive histidine–polyphosphate interaction","year":2024,"lang":"en","type":"article","venue":"Protein Science","topic":"Coagulation, Bradykinin, Polyphosphates, and Angioedema","field":"Medicine","cited_by":8,"is_retracted":false,"has_abstract":true,"ca_institutions":"Queen's University","funders":"Distinguished Young Scholar Foundation of Hunan Province; Natural Science Foundation for Distinguished Young Scholars of Hunan Province; Cystic Fibrosis Canada","keywords":"Nitrilotriacetic acid; Histidine; Polyphosphate; Chemistry; Tandem affinity purification; Protein purification; Affinity chromatography; Biochemistry; Chromatography; Chelation; Recombinant DNA; Combinatorial chemistry; FLAG-tag; Amino acid; Organic chemistry; Fusion protein; Enzyme","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003618395,0.0006581063,0.0004406825,0.0002607733,0.0003310908,0.0003135868,0.0004158133,0.0003787365,0.0009683837],"category_scores_gemma":[0.0003931947,0.000239691,0.0003694744,0.0003887365,0.000226372,0.000353715,0.0004758571,0.000847691,0.001078149],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002386006,"about_ca_system_score_gemma":0.0002623354,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0006893661,"about_ca_topic_score_gemma":0.001151941,"domain_scores_codex":[0.9995579,0.00006627643,0.00004904487,0.0001235153,0.0001351719,0.00006806842],"domain_scores_gemma":[0.9998302,0.0000382903,0.00003517326,0.00002601193,0.00004830714,0.00002199173],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003530021,0.00002279999,0.0001134296,0.0001285735,0.0000102026,0.00007231737,0.00002723321,0.00003816564,0.9961134,0.00009993865,0.0001614187,0.003177175],"study_design_scores_gemma":[0.000006835657,0.00009349515,0.001656325,0.000008794955,0.00001712478,0.0004192872,0.00001444226,0.0009327856,0.9888987,0.00007600848,0.007867486,0.000008742672],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.717217,0.008358066,0.2635029,0.0007648646,0.0003106337,0.0007770365,0.001412631,0.001039343,0.006617439],"genre_scores_gemma":[0.7762726,0.006435408,0.1961774,0.0005973123,0.00009065648,0.0005993922,0.005151946,0.000238685,0.01443666],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0009683837,"threshold_uncertainty_score":0.003239632,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02309806316392053,"score_gpt":0.3059200874247764,"score_spread":0.2828220242608559,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}