{"id":"W4396927795","doi":"10.1002/pro.5021","title":"Protein purification via consecutive histidine–polyphosphate interaction","year":2024,"lang":"en","type":"article","venue":"Protein Science","topic":"Coagulation, Bradykinin, Polyphosphates, and Angioedema","field":"Medicine","cited_by":8,"is_retracted":false,"has_abstract":true,"ca_institutions":"Queen's University","funders":"Distinguished Young Scholar Foundation of Hunan Province; Natural Science Foundation for Distinguished Young Scholars of Hunan Province; Cystic Fibrosis Canada","keywords":"Nitrilotriacetic acid; Histidine; Polyphosphate; Chemistry; Tandem affinity purification; Protein purification; Affinity chromatography; Biochemistry; Chromatography; Chelation; Recombinant DNA; Combinatorial chemistry; FLAG-tag; Amino acid; Organic chemistry; Fusion protein; Enzyme","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0007581098,0.0001960243,0.0002049834,0.0003269824,0.0002883654,0.0002106621,0.000201045,0.00005985731,0.0002721875],"category_scores_gemma":[0.000343351,0.0001681336,0.00009306052,0.001256717,0.000574736,0.0007699472,0.0000613442,0.0002216338,0.0004017564],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003022437,"about_ca_system_score_gemma":0.0005776048,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0002456222,"about_ca_topic_score_gemma":0.000007932652,"domain_scores_codex":[0.9979113,0.00003236059,0.0003640854,0.0006697951,0.0006286199,0.0003938494],"domain_scores_gemma":[0.9989994,0.00004307874,0.0001080282,0.0003686266,0.0002671545,0.0002136818],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001405096,0.0001085367,0.0006592408,0.0002163832,0.00002381545,0.00005680948,0.0009627181,0.000005720667,0.8738239,0.0224701,0.0002924055,0.1012398],"study_design_scores_gemma":[0.0004326718,0.0003089718,0.006740965,0.001041228,0.00004910798,0.0001773821,0.0001653826,0.01956476,0.9381061,0.003921989,0.02916076,0.0003307211],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9498432,0.005258581,0.0245944,0.006664219,0.001202855,0.003726499,0.00001238363,0.0007875699,0.00791024],"genre_scores_gemma":[0.9902598,0.00001537758,0.003232677,0.0001356478,0.000202438,0.0002848869,0.00001310923,0.00002377614,0.005832313],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1009091,"threshold_uncertainty_score":0.6856288,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02309806316392053,"score_gpt":0.3059200874247764,"score_spread":0.2828220242608559,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}