{"id":"W4397009525","doi":"10.12688/f1000research.150684.1","title":"A guide to selecting high-performing antibodies for Protein-glutamine gamma-glutamyltransferase 2 (TGM2) for use in western blot, immunoprecipitation and immunofluorescence","year":2024,"lang":"en","type":"preprint","venue":"F1000Research","topic":"Erythrocyte Function and Pathophysiology","field":"Medicine","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University; Montreal Neurological Institute and Hospital","funders":"Canadian Institutes of Health Research; Mitacs; Ontario Genomics; Genome Canada; Michael J. Fox Foundation for Parkinson's Research","keywords":"Western blot; Immunoprecipitation; Plant biology; Immunofluorescence; Open peer review; Antibody; Blot; Biology; Immunology; Biochemistry; Botany","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.006674651,0.00424571,0.003097635,0.006764363,0.00170295,0.002418336,0.005571835,0.003513134,0.09812865],"category_scores_gemma":[0.0107601,0.005617729,0.002066611,0.003601672,0.00136207,0.002165741,0.001881463,0.006256565,0.157836],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.001327567,"about_ca_system_score_gemma":0.002073093,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001605327,"about_ca_topic_score_gemma":0.004489614,"domain_scores_codex":[0.9951642,0.001638957,0.0007821615,0.0005328126,0.001477277,0.0004046345],"domain_scores_gemma":[0.9930043,0.002728147,0.0003405976,0.001594648,0.001822483,0.000509875],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"not_applicable","study_design_gemma":"not_applicable","study_design_scores_codex":[0.001006778,0.0005106712,0.0008206553,0.003294339,0.0001484856,0.0009893334,0.0003554084,0.0009843323,0.3298587,0.005393284,0.5499577,0.1066804],"study_design_scores_gemma":[0.0002565212,0.0002818022,0.002397195,0.0004331899,0.00007023715,0.00178075,0.00008142619,0.001170022,0.05719563,0.003683561,0.9325488,0.000100962],"study_design_candidate":"not_applicable","study_design_consensus":"not_applicable","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.008249783,0.01626417,0.7360607,0.007597071,0.004684615,0.008775439,0.07665216,0.07137999,0.0703361],"genre_scores_gemma":[0.008349074,0.01227447,0.8029491,0.003108074,0.0009745783,0.01732064,0.08270355,0.0140781,0.05824234],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.09812865,"threshold_uncertainty_score":0.328273,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.04562137948250439,"score_gpt":0.3628079351269788,"score_spread":0.3171865556444744,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}