{"id":"W4398349745","doi":"10.21203/rs.3.rs-4155503/v1","title":"Integrated bioprocessing and genetic strategies to enhance soluble expression of anti-HER2 immunotoxin in E. coli","year":2024,"lang":"en","type":"preprint","venue":"Research Square","topic":"Toxin Mechanisms and Immunotoxins","field":"Immunology and Microbiology","cited_by":0,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Waterloo","funders":"","keywords":"Bioprocess; Immunotoxin; Chemistry; Computational biology; Biology; Biochemistry; Cytotoxicity; In vitro","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002551176,0.0004973851,0.0003945638,0.0003181442,0.0001234853,0.0007863873,0.0002728335,0.000507624,0.000674348],"category_scores_gemma":[0.0002614866,0.0001819315,0.0003830905,0.0004462401,0.0002488922,0.0002892507,0.0003428406,0.0008035398,0.0004265908],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004012949,"about_ca_system_score_gemma":0.0002129706,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004957427,"about_ca_topic_score_gemma":0.0007738316,"domain_scores_codex":[0.9997438,0.00003240833,0.00002442395,0.00004388465,0.0001026574,0.00005286427],"domain_scores_gemma":[0.9998953,0.00002222484,0.00003143413,0.00002215712,0.00001512989,0.00001362917],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003213836,0.000044272,0.00005323798,0.00001998299,0.000004051416,0.00002083503,0.00000667786,0.0002161058,0.9983276,0.0001024087,0.00002556572,0.001147201],"study_design_scores_gemma":[0.000006160555,0.00006541003,0.0003346004,0.00000254231,0.000007021445,0.00003702144,0.000007371982,0.001060837,0.9977304,0.00004699784,0.000698441,0.000003172622],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9677936,0.0008879537,0.02881125,0.0002345826,0.00008921514,0.00008248266,0.0005243895,0.0002474744,0.001329041],"genre_scores_gemma":[0.9592426,0.00104425,0.03310047,0.0001260751,0.00002083612,0.00008273556,0.00114362,0.0001125855,0.005126841],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0007863873,"threshold_uncertainty_score":0.002911568,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0295831316974368,"score_gpt":0.3586103555980609,"score_spread":0.3290272239006241,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}