{"id":"W4399035655","doi":"10.1080/15548627.2024.2356490","title":"Development and characterization of phospho-ubiquitin antibodies to monitor PINK1-PRKN signaling in cells and tissue","year":2024,"lang":"en","type":"article","venue":"Autophagy","topic":"Connective tissue disorders research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":12,"is_retracted":false,"has_abstract":true,"ca_institutions":"ImmunoPrecise (Canada)","funders":"Institute of Aging; National Institute of Neurological Disorders and Stroke; National Institute on Aging; Ted Nash Long Life Foundation; Wellcome Trust; Congressionally Directed Medical Research Programs; Mayo Clinic; Florida Department of Health; Michael J. Fox Foundation for Parkinson's Research; American Parkinson Disease Association; U.S. Department of Defense","keywords":"Biology; PINK1; Ubiquitin; Cell biology; Antibody; Ubiquitin-Protein Ligases; Signal transduction; Ubiquitin ligase; Autophagy; Immunology; Mitophagy; Genetics; Apoptosis","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0007755259,0.001026178,0.0004261224,0.0006749643,0.0002582501,0.0003783961,0.0006656617,0.0005965497,0.001009037],"category_scores_gemma":[0.0004956704,0.0004127885,0.000439787,0.000489679,0.0002146168,0.0004519239,0.0002811811,0.000852617,0.00117473],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004787354,"about_ca_system_score_gemma":0.0003718985,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000654618,"about_ca_topic_score_gemma":0.001380765,"domain_scores_codex":[0.9994381,0.00009572074,0.00008222485,0.000124037,0.0001604492,0.0000994107],"domain_scores_gemma":[0.9995272,0.00006919706,0.0000952174,0.00007088154,0.000171495,0.00006604782],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001490614,0.00002563069,0.00006837837,0.00003007112,0.000003537815,0.00001832352,0.000008374501,0.00005601531,0.9986886,0.0000400734,0.00002788839,0.001018162],"study_design_scores_gemma":[0.00002160587,0.0003345307,0.002486176,0.00001301666,0.00003261387,0.00041692,0.00001846064,0.001199347,0.9890592,0.00005681773,0.006353777,0.000007401492],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.7473793,0.009195047,0.2306548,0.0004984798,0.0001525449,0.001771079,0.002670806,0.0007311716,0.006946799],"genre_scores_gemma":[0.7213343,0.008790847,0.2470992,0.0005509201,0.00008109334,0.001953972,0.01109452,0.0002652439,0.008829972],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001026178,"threshold_uncertainty_score":0.004101455,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01172739034100371,"score_gpt":0.2886602381889349,"score_spread":0.2769328478479312,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}