{"id":"W4399035655","doi":"10.1080/15548627.2024.2356490","title":"Development and characterization of phospho-ubiquitin antibodies to monitor PINK1-PRKN signaling in cells and tissue","year":2024,"lang":"en","type":"article","venue":"Autophagy","topic":"Connective tissue disorders research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":12,"is_retracted":false,"has_abstract":true,"ca_institutions":"ImmunoPrecise (Canada)","funders":"Institute of Aging; National Institute of Neurological Disorders and Stroke; National Institute on Aging; Ted Nash Long Life Foundation; Wellcome Trust; Congressionally Directed Medical Research Programs; Mayo Clinic; Florida Department of Health; Michael J. Fox Foundation for Parkinson's Research; American Parkinson Disease Association; U.S. Department of Defense","keywords":"Biology; PINK1; Ubiquitin; Cell biology; Antibody; Ubiquitin-Protein Ligases; Signal transduction; Ubiquitin ligase; Autophagy; Immunology; Mitophagy; Genetics; Apoptosis","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001174119,0.00007938678,0.00009842123,0.00008001962,0.00002357801,0.00002571522,0.00004743437,0.00005309916,0.00001501418],"category_scores_gemma":[0.00002254433,0.00008235613,0.000008581741,0.00009647159,0.0000304347,0.000004398594,0.0001063753,0.00004105379,0.000005040553],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000009356834,"about_ca_system_score_gemma":0.00003558638,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00002701768,"about_ca_topic_score_gemma":0.00002181409,"domain_scores_codex":[0.9994112,0.00002190368,0.000131029,0.0002344862,0.00007113126,0.0001302425],"domain_scores_gemma":[0.9998289,0.00001342049,0.00001527177,0.00007115307,0.00003326474,0.00003794338],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001795441,0.00001334109,0.000428191,0.00009421854,0.00001343973,0.000007288188,0.0007756482,0.000001942896,0.933929,0.00004013495,0.00001109869,0.06466774],"study_design_scores_gemma":[0.0001113713,0.00007560245,0.01844046,0.00005790341,0.000002328631,0.000002051295,0.000110533,0.00005784705,0.970597,0.00001229728,0.0104457,0.00008685919],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9970475,0.002209834,0.0003613335,0.00007654884,0.00005386521,0.0001770247,0.00001123615,0.000007294585,0.00005531889],"genre_scores_gemma":[0.998546,0.0002357117,0.0007000254,0.00002580349,0.00002937388,0.00001814574,0.00003264176,0.00001251207,0.0003997586],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.06458088,"threshold_uncertainty_score":0.3358386,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01172739034100371,"score_gpt":0.2886602381889349,"score_spread":0.2769328478479312,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}