{"id":"W4399354845","doi":"10.1093/plphys/kiae316","title":"Development of a rapamycin-inducible protein-knockdown system in the unicellular red alga <i>Cyanidioschyzon merolae</i>","year":2024,"lang":"en","type":"article","venue":"PLANT PHYSIOLOGY","topic":"Algal biology and biofuel production","field":"Energy","cited_by":11,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"JST-Mirai Program; Institute of Genetics; RIKEN; Japan Science and Technology Agency; Japan Society for the Promotion of Science; Tokyo Metropolitan University","keywords":"Skp1; Gene knockdown; Cell biology; Ubiquitin ligase; Biology; Cullin; RNA interference; E2F; FKBP; Autophagy-related protein 13; Cell Cycle Protein; Protein degradation; Ubiquitin; Protein kinase A; Transcription factor; Biochemistry; Kinase; Gene; Cell cycle; RNA; Cyclin-dependent kinase 2","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00022538,0.0005372228,0.0003162291,0.0002638866,0.0003389956,0.000309628,0.0005054623,0.0003427926,0.0008984447],"category_scores_gemma":[0.00009004559,0.0002812685,0.0003486942,0.0001836639,0.0002438677,0.0002938392,0.0003380594,0.0006863038,0.0006219622],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000529971,"about_ca_system_score_gemma":0.0004848072,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002257045,"about_ca_topic_score_gemma":0.003275156,"domain_scores_codex":[0.9998069,0.00002231139,0.00003135781,0.00005793135,0.00005964752,0.00002195704],"domain_scores_gemma":[0.9998674,0.00002082143,0.00004114446,0.0000244548,0.00001920501,0.00002701304],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000010395,0.000007592515,0.00005002279,0.00002382939,0.000002451753,0.00001363445,0.000008268309,0.00002720182,0.9992218,0.00008471739,0.00003239274,0.0005177535],"study_design_scores_gemma":[0.00001154618,0.00006191727,0.001989373,0.000004530459,0.00001636269,0.0001089575,0.00001133139,0.0009364221,0.9920631,0.0000175034,0.004772015,0.000006884817],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9045291,0.002250074,0.08052017,0.0004992281,0.0002133882,0.000682563,0.00364989,0.001767036,0.005888479],"genre_scores_gemma":[0.9030353,0.002114764,0.07521347,0.0002255362,0.00003104467,0.0007723534,0.005868131,0.0004283906,0.01231104],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002257045,"threshold_uncertainty_score":0.004487813,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01790571940455935,"score_gpt":0.2140301734472293,"score_spread":0.19612445404267,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}