{"id":"W4399496619","doi":"10.17504/protocols.io.rm7vzj362lx1/v3","title":"DENV2 NS2B-NS3 protease co-expression construct small scale expression and purification protocol v2","year":2024,"lang":"en","type":"preprint","venue":"","topic":"Ubiquitin and proteasome pathways","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"Discovery Centre","funders":"","keywords":"Construct (python library); Protease; Expression (computer science); Scale (ratio); Protocol (science); Biology; Computational biology; Computer science; Enzyme; Biochemistry; Geography; Cartography; Computer network","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0007452122,0.001535004,0.001469054,0.001296606,0.001340077,0.001203265,0.001598153,0.0008281411,0.0230156],"category_scores_gemma":[0.0006505793,0.001261589,0.0008873673,0.00145449,0.0004511216,0.0006002523,0.001167434,0.003040491,0.03625144],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0008917336,"about_ca_system_score_gemma":0.0008482129,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002101933,"about_ca_topic_score_gemma":0.002994315,"domain_scores_codex":[0.9992224,0.00008884791,0.00006219916,0.0001691655,0.000310144,0.0001471796],"domain_scores_gemma":[0.9997323,0.00004877214,0.00001478565,0.00009501912,0.00006410293,0.00004494438],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"not_applicable","study_design_scores_codex":[0.0004311536,0.0001699227,0.0003230112,0.0007361415,0.00007388313,0.000188457,0.0001608807,0.0003880929,0.9311007,0.003681703,0.04819824,0.01454786],"study_design_scores_gemma":[0.0000789643,0.00007906438,0.001209439,0.00005601678,0.00005864717,0.0005450413,0.00004660489,0.00156562,0.7172648,0.001249502,0.2777888,0.00005743308],"study_design_candidate":"not_applicable","study_design_consensus":null,"genre_codex":"methods","genre_gemma":"protocol","genre_scores_codex":[0.1212494,0.006274291,0.5303429,0.00233513,0.002745222,0.008109356,0.2402036,0.02483293,0.06390712],"genre_scores_gemma":[0.1123411,0.005532079,0.2707486,0.0007318069,0.0002364207,0.007900424,0.473967,0.007841881,0.1207007],"genre_candidate":"protocol","genre_consensus":null,"teacher_disagreement_score":0.0230156,"threshold_uncertainty_score":0.07699484,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02122461074120013,"score_gpt":0.2903289814645023,"score_spread":0.2691043707233021,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}