{"id":"W4399505093","doi":"10.1158/1538-8514.synthleth24-a005","title":"Abstract A005: A live cell PRMT5 NanoBRET™ target engagement assay querying competitive and uncompetitive modes of inhibition","year":2024,"lang":"en","type":"article","venue":"Molecular Cancer Therapeutics","topic":"Cancer-related gene regulation","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"","keywords":"Protein arginine methyltransferase 5; Uncompetitive inhibitor; Methylation; Methyltransferase; Chemistry; Biochemistry; Non-competitive inhibition; Enzyme; Cell biology; Biology; DNA","routes":{"ca_aff":false,"ca_fund":false,"ca_venue":false,"about_ca":true,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.0002454959,0.0002379679,0.00019437,0.00008647465,0.00006980367,0.00004593888,0.00007953169,0.0001862925,0.00005931244],"category_scores_gemma":[0.000006417275,0.000245792,0.0001158475,0.0001396166,0.000134314,0.0000114678,0.00008332476,0.0001911452,0.000004047612],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00009921915,"about_ca_system_score_gemma":0.0001922057,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00007824438,"about_ca_topic_score_gemma":0.00004698917,"domain_scores_codex":[0.9986951,0.0001256693,0.0002713851,0.0004468455,0.0002274429,0.0002335776],"domain_scores_gemma":[0.9994394,0.00002595282,0.0001143216,0.0002162636,0.0001475522,0.00005646759],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00007756554,0.00005054863,0.0001133136,0.0002022938,0.0003100037,0.00001110879,0.0004409775,0.004554737,0.9868303,0.001143703,0.00007375596,0.006191719],"study_design_scores_gemma":[0.0004493578,0.000194334,0.0003742001,0.0002895565,0.0001283258,0.000005802945,0.0003522421,0.002342541,0.9722319,0.000360928,0.02298329,0.0002874745],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.911265,0.06045896,0.02348462,0.0004871101,0.000356475,0.0006232595,0.0002232814,0.0000516433,0.003049619],"genre_scores_gemma":[0.9953869,0.002791299,0.001092251,0.0001990668,0.0001337941,0.00004715016,0.0001571171,0.00005622229,0.0001362171],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.08412186,"threshold_uncertainty_score":0.9999994,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0137668169459198,"score_gpt":0.2651102916968234,"score_spread":0.2513434747509036,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}