{"id":"W4399505093","doi":"10.1158/1538-8514.synthleth24-a005","title":"Abstract A005: A live cell PRMT5 NanoBRET™ target engagement assay querying competitive and uncompetitive modes of inhibition","year":2024,"lang":"en","type":"article","venue":"Molecular Cancer Therapeutics","topic":"Cancer-related gene regulation","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"","keywords":"Protein arginine methyltransferase 5; Uncompetitive inhibitor; Methylation; Methyltransferase; Chemistry; Biochemistry; Non-competitive inhibition; Enzyme; Cell biology; Biology; DNA","routes":{"ca_aff":false,"ca_fund":false,"ca_venue":false,"about_ca":true,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00108566,0.00134037,0.0009241192,0.0006925898,0.0004546577,0.0008937449,0.001419139,0.001372742,0.005322392],"category_scores_gemma":[0.0006601693,0.0005113693,0.0005671991,0.0009193171,0.0005401583,0.0006951903,0.0005973054,0.001767387,0.001918585],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0009834152,"about_ca_system_score_gemma":0.0005224055,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002120018,"about_ca_topic_score_gemma":0.002615599,"domain_scores_codex":[0.9976877,0.0005514764,0.0001665956,0.0004606872,0.0009642629,0.0001692275],"domain_scores_gemma":[0.9994236,0.000223091,0.00008321185,0.0000719391,0.0001414007,0.0000568444],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000175726,0.0001658267,0.0002377515,0.0001696661,0.00002097211,0.00005028314,0.00006992263,0.0003584557,0.9922954,0.000651138,0.0006574402,0.005147402],"study_design_scores_gemma":[0.0000340568,0.0005197967,0.0008565338,0.00002054155,0.00002451338,0.0001106851,0.00003531647,0.006054191,0.9879281,0.0001624367,0.004233651,0.00002020275],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.6114891,0.00726138,0.3227931,0.0009639298,0.0005179981,0.001115793,0.006941841,0.004499454,0.04441743],"genre_scores_gemma":[0.7953617,0.003123822,0.1596428,0.0004907212,0.0000939574,0.001331042,0.009471852,0.0003538109,0.03013038],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.005322392,"threshold_uncertainty_score":0.01780522,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0137668169459198,"score_gpt":0.2651102916968234,"score_spread":0.2513434747509036,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}