{"id":"W4399630629","doi":"10.1128/aem.00699-24","title":"A CRISPR-Cas-associated transposon system for genome editing in <i>Burkholderia cepacia</i> complex species","year":2024,"lang":"en","type":"article","venue":"Applied and Environmental Microbiology","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":10,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Manitoba","funders":"Natural Sciences and Engineering Research Council of Canada; Canadian Institutes of Health Research; Cystic Fibrosis Canada; University of Manitoba; Research Manitoba","keywords":"Transposase; Biology; CRISPR; Burkholderia cepacia complex; Genome editing; Genetics; Computational biology; Genome; Burkholderia cenocepacia; Transposable element; Insertional mutagenesis; Forward genetics; Transposon mutagenesis; Burkholderia; Cas9; Gene; Bacteria","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003535488,0.000512952,0.0003125666,0.000385532,0.0002465436,0.0004209218,0.000469746,0.0005280763,0.001380773],"category_scores_gemma":[0.0002852015,0.0002472178,0.000354787,0.0002145788,0.0002371548,0.0002281962,0.0004881465,0.000743141,0.0006028254],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003406896,"about_ca_system_score_gemma":0.0003102462,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001054234,"about_ca_topic_score_gemma":0.001155151,"domain_scores_codex":[0.9996433,0.00006308441,0.00005344299,0.00008234863,0.0001025579,0.00005529743],"domain_scores_gemma":[0.9997774,0.0000432964,0.00008057459,0.00003680656,0.0000252266,0.00003671632],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000024436,0.00001980001,0.0000959438,0.00003220378,0.000006053902,0.00006606641,0.00001346111,0.0001388393,0.9976065,0.0001935416,0.00008853368,0.001714705],"study_design_scores_gemma":[0.00002077205,0.0001689823,0.001378387,0.00001377973,0.00002309927,0.0003953202,0.00002926905,0.004896116,0.9845248,0.0001213351,0.008407216,0.00002088597],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8356012,0.001492031,0.1508256,0.000460569,0.0002334941,0.0005113164,0.002166809,0.003085333,0.005623638],"genre_scores_gemma":[0.9346598,0.0004407949,0.05857448,0.0001253567,0.00001349121,0.0001603388,0.001512495,0.0002008604,0.004312448],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001380773,"threshold_uncertainty_score":0.004619122,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006589535812835965,"score_gpt":0.2190120994855436,"score_spread":0.2124225636727077,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}